Competitive vs non-competitive assay

Question 1

what non-competitive assay

Answer 1

in this assay antigen is captured by large number of binding site (antibody) before is detected

Question 2

what is the benefit of using two antibody

Answer 2

it increase specificity

Question 3

what is the principle of non-competitive analysis

Answer 3

• you first coat with specific antibody in high concentration
• then add sample in high concentration

-then add enzyme labelled specific antibody (secondary antibody) in high concentration

Question 4

why do we have to add sample in high concentration

Answer 4

in order to get high or more signal

Question 5

competitive analysis

Answer 5

is when labeled antigen and un-labeled antigen compete for limited number of binding site

Question 6

what is the component of competitive analysis

Answer 6

coat with specific antibody in low concentration
labeled antigen and non-labeled antigen/ sample

Question 7

what is the benefit of using one antibody in competitive analysis

Answer 7

it increase specificity

Question 8

what is the benefit of adding sample (labelled and un-labeled antigen) in low concentration in competitive analysis

Answer 8

it increase signal because labelled antigen has few unlabeled antigen to compete with

Question 9

compare antigen concentration in non-competitive and competitive analysis

Answer 9

non-competitive analysis: antigen concentration increase with increase of signal

competitive analysis: antigen concentration increase with decrease of signal

Question 10

what are the application of non-competitive and competitive analysis

Answer 10

non-competitive analysis : useful for higher molecular weight antigen only. do not require access to pure antigen. do not depends to affinity

competitive analysis: can be used for both low molecular weight antigen and higher molecular weight antigen. require access to pure antigen. depends to affinity