Retro Flashcards
Retrovirus structure
large, enveloped
ss (+)RNA
Retrovirus is the only virus to contain _______
diploid RNA genome
Retrovirus enzymes
RT
integrase
protease
3 Retrovirus genera that infect humans
Lentivirus
Oncovirus (HTLV)
Spumavirus
Lentivirus properties (3)
Bodily fluid transmission
non-transforming
have CD4+ T cell tropism
HIV believed to be native to
Monkeys of W Africa
HIV genome structure and contents
2 identical +RNA strands consisting of 3 major genes
- Gag
- Pol
- Env
HIV gag protein function
capsid and matrix
HIV Pol function
polymerase
core contains RT, integrase, and protease
HIV Env function
Envelope (glycoproteins)
HIV glycoproteins, both encoded by ENV gene, are ___ and ___.
gp120 (attachment)
gp41 (fusion)
HIV genome is +RNA, BUT….
it is NOT infectious
(because it does not encode a polymerase that directly generates more mRNA)
HIV attachment and binding (4) steps…
- gp120 -> CD4
- CCR5 (CXCR4) recruitment
- gp41 inserts into membrane
- fusion
Steps following HIV attachement (5)
Initial copies of viral DNA made, (2) dsDNA inserted into the host cell chromosome and the host cell RNA POL is used to make viral RNA. (3) New RNA strands serve as templates and as mRNA. (4) mRNA translated to viral proteins to make envelope. (5) New viral particles bud off
HIV: primary stage features
short flu-like illness
(OR asymptomatic)
Infecter person CAN infect others