Respiratory Flashcards
What are the two conformations of hemoglobin?
T-State: Low oxygen affinity
R-State: High oxygen affinity
What stabilizes hemoglobin into the T-state?
- H+
- CO2
- 2,3-DPG
*Due to products of processes occurring in the tissues → in the tissues we want to offload oxygen (low affinity)
What stabilizes hemoglobin in the R-state?
- O2
2. CO
What happens in carbon monoxide poisoning?
The R-form of hemoglobin is stabilized. CO binds to hemoglobin with a higher affinity than oxygen
How does the O2-hemoglobin curve shift in acidemia
Right
How does the O2-hemoglobin curve shift with increased CO2 concentrations?
Right
How does the O2-hemoglobin curve shift in decreased temperatures?
Left
How does the O2-hemoglobin curve shift in increased 2,3-DPG?
Right
What is the Bohr effect?
The effect of CO2 and H+ on the affinity of hemoglobin for O2
- Increased CO2 and H+ → decreases affinity/promotes offloading
- Decreasing CO2 and H+ → increases affinity/enhances loading
How does the O2-hemoglobin curve shift at the pulmonary capillary bed?
Left
- Decreased p50
- Increased O2 affinity and uptake
What forms does CO2 exist in the blood?
- HCO3 - 70%
- Hemoglobin - 23%
- Dissolved in plasma (pCO2) - 7%
How does CO2 interact with the red blood cells?
It is brought into the red blood cell and interacts with carbonic anhydrase → ultimately results in the dissociation of carbonic acid into H+ and HCO3 → HCO3 diffuses out of the red blood cell and Cl shifts in (Chloride shift)
What is the Haldane effect?
O2 + hemoglobin = stronger acid
- Less tendency to combine with CO2 to form carbaminohemoglobin
- Release an excess of H2 ions
What is the difference between the Bohr and Haldane effects?
- Bohr → increase in CO2 in blood causes O2 to be displaced from oxygen (tissues)
- Haldane → binding of O2 with hemoglobin causes CO2 to be displaced from the hemoglobin (lungs)
What determines the excretion of CO2?
Minute ventilation (TV x RR)
What are the regulators of respiration (what and where)?
- Central chemoreceptors - CO2
- 2/3 of CO2 regulation (slow) - Peripheral chemoreceptors - CO2
- 1/3 of CO2 regulation (fast)
Where are the peripheral chemoreceptors?
Aortic and carotid bodies
How do peripheral chemoreceptors detect decreases in PO2? What happens when decreased PO2 detected?
Glomus cells → O2 sensitive K channels
Decreased O2 → K efflux → calcium influx → depolarization → dopamine release → respiratory stimulation via cranial nerve IX
Why does CO2 have a more potent effect in stimulating the central chemoreceptor neurons than hydrogen ions?
The blood brain barrier is not very permeable to hydrogen ions, but CO2 passes easily through the BBB
Where are the central chemoreceptors located?
Chemosensitive area bilaterally of the medulla oblongata
How do the central chemoreceptors work?
Excited more easily by hydrogen ions → problem because H does not easily pass the BBB
CO2 passes through the BBB and interacts with carbonic anhydrase to form H+ and HCO2 → the H+ then interacts with the central chemoreceptor
Where is the respiratory center located?
Bilaterally in the medulla oblongata and the pons
What are the different groups in the respiratory center? What do they control?
- Dorsal respiratory group → Inspiration
- Ventral respiratory group → Expiration (and inspiration), inactive during normal quiet respiration
- Pneumotaxic center → rate and depth of breathing, the inspiratory “off-switch”
What disease is associated with dysfunctional cilia?
Primary cilia dyskinesia (PCD)
What breed is predisposed to primary cilia dyskinesia?
Bichon Frise
*Old English Sheepdogs, Alaskan malamutes and English pointers can also be affected
What are the clinical signs associated with primary cilia dyskinesia?
- Nasal discharge
- Recurrent respiratory infections
- Male infertility
- Hydrocephalus
- Sinus inversus
What is the cause of primary cilia dyskinesia?
Mutation in microtubule conformations
Parasympathetic ACh causes what effect in the lung?
BronchoCONSTRICTION
Sympathetic Epi/Norepi causes what effect in the lung? Via which receptor?
BronchoDILATION
- B2 adrenergic receptors
What is the most common form of canine fungal rhinitis? How is this treated?
Aspergillus
Tx: Clotrimazole infusion
What is the most common form of feline fungal rhinitis? How is this treated
Cryptococcus
Tx: Fluconazole
What compose the conducting airways?
Trachea and bronchi
*Anatomic dead space
What are the basal cells in the respiratory epithelium?
- Lie underneath the columnar cells
- Stem cell producing new epithelial and goblet cells
- ABSENT in bronchioles and beyond
What cells secrete secrete surfactant?
Type II alveolar epithelial cell (granular pneumocytes)
Which alveolar epithelial cells are most abundant?
Type 1 - 95%
Type 2 - 5%
What are the roles of type 1 alveolar epithelial cells (pneumocytes)
- Line alveoli
- Function in gas exchange
- Do not divide in vivo
*Exposed to the highest concentration of oxygen - can. have oxygen toxicity damage
What are the roles of type 2 alveolar epithelial cells (pneumocytes)
- Stem cells from which type 1 cells arise
- No gas exchange
- Produces/Stores surfactant in the cytoplasm
- Resistant to oxygen toxicity damage
What is surfactant composed of?
- 80% phospholipids
- 5-10% neutral lipids
- 8-10% proteins
What muscle(s) plays a role in the process of inspiration?
- Diaphragm → contracts and flattens, causes intrapleural space to become more negative
- External intercostals → make diaphragm contraction more efficient, little role at rest, greater role during exercise
What muscle(s) plays a role in the process of expiration?
- Typically a passive process*
1. Abdominal muscles push diaphragm up → increase the intrapleural pressure
2. Internal intercostals → oppose action of external intercostals, pull ribcage down and in
3. Accessory muscles → laryngeal muscles, act as “breaks”
What components determine lung compliance?
- Elastic forces of the lung → 1/3, composed of elastin and collagen fibers
- Elastic forces caused by surface tension of the fluid that lines the inside of the alveoli → 2/3, surfactant
What decreases lung compliance?
- High lung volume
- Surfactant deficiency
- Pulmonary edema
- Atelectasis or alveolar collapse
- Pulmonary fibrosis
- Smooth muscle constriction in the small airways (asthma)
What increases lung compliance?
- Age
- Emphysema
- Body size
Which breed is predisposed to idiopathic pulmonary fibrosis?
Westies!
What is tidal volume?
Volume of air inspired or expired with each normal breath
10-15 ml/kg
What is the inspiratory reserve volume?
The extra volume of air that can be inspired OVER the normal tidal volume
What is the inspiratory capacity?
Tidal volume + inspiratory reserve volume
The total amount of air that an individual can breathe in
What is the expiratory reserve volume?
The extra volume of air that can be expelled by an active expiratory effort AFTER passive expiration
What is the the residual volume?
The volume of air remaining in the lungs after the most forceful expiration
What is the functional residual capacity?
Expiratory reserve volume + residual reserve volume
The amount of air that remains in the lungs at the end of normal expiration
What is the vital capacity?
Tidal volume + inspiratory reserve volume + expiratory reserve volume
The maximum amount of air an individual can expel from the lungs after filling the lungs to the maximum extent and then expiring to a maximum extent
What is the total lung capacity?
Vital capacity + residual volume
The maximum amount of air the lungs can be expanded with the greatest amount of effort
What factors affect diffusion in the lung?
- Thickness of membranes
- Surface area
- Diffusion coefficient of gas
- Partial pressure difference
What happens to the blood vessel if the concentration of O2 in an alveoli decreases?
- Adjacent blood vessels constrict → increase vascular resistance
- Distributes blood flow to where the lungs are better aerated
What occurs to V/Q in the following scenarios?
- Without ventilation
- Without perfusion
- V/Q = 0
2. V/Q = infinity
What diseases can lead to a low V/Q (poor ventilation, decreased PaO2)
- Chronic bronchitis
- Asthma
- Pulmonary edema
*Ventral lungs have slightly lower V/Q
What diseases can lead to a high V/Q (poor perfusion, increased PaCO2)
- Pulmonary thromboembolism
* Dorsal lungs have high V/Q
Which intestinal worms have pulmonary migration?
- Ancylostoma
2. Toxocara
How do you treat intestinal worm associated pulmonary disease?
- Glucocorticoids
2. Fenbendazole - 2 doses, 2 weeks apart
Lung worms are (easy/difficult) to diagnose based on fecal flotation or sedimentation. Why?
- Difficult
- Intermittent shedding
*Treat with empiric fenbendazole if suspected
What is the most common feline lungworm? Where (geographically) is it most prevalent?
- Aelurostrongylus
2. Southern US
Where do Aelurostrongylus reside in the lungs?
Terminal bronchioles and alveoli
How are Aelurostrongylus transmitted?
- Fecal-oral transmission
* They are coughed up and then passed in the feces - Eating encysted larvae in the tissue of mice and birds
What are the clinical signs associated with Aelurostrongylus infection?
- Mild
- Severe
- Typically asymptomatic - Hard to differentiate from feline lower airway disease!
- Severe eosinophilic bronchopneumonia with bronchointerstitial to alveolar infiltrates
How is Aelurostrongylus diagnosed?
BAL or Baerman
How do you treat an Aelurostrongylus infection?
- Fenbendazole or macrocyclic lactone
- Anti-inflammatory glucocorticoids
- Bronchodilators
What are the lung flukes? Where are they present geographically?
- Paragonimus kellicoti and westermani
2. Great Lakes, midwest, and South
What do paragonimus sp. cause in the lungs of dogs and cats?
Cysts
What clinical signs are common with paragonimus sp.
- Eosinophilic pleurites and cough (common)
- Nodules, bullae, and atelectasis can occur in the RIGHT MIDDLE LUNG LOBE
- Hemoptysis and pneumothorax can occur when adults emerge
How are paragonimus sp. diagnosed?
- Ova often present on BAL
2. Intermittently present on sedimentation
How are animals infected with paragonimus sp.?
Eating raw crabs or crayfish
How are paragonimus sp. treated?
Fenbendazole or praziquantel
What are the canine lungworms?
- Filaroides hirthi and milksi
2. Crenosoma vulpis
What population acquires filaroides spp.?
Research colonies
Adult filaroides live in which part of the lung?
Terminal bronchioles and alveoli
How are filaroides sp. treated?
Fenbendazole or ivermectin
Where is crenosoma vulpis located geographically?
- Northeastern US
2. Canada
Where are crenosoma vulpis adults located in the lung?
Terminal bronchioles and alveoli
How are crenosoma vulpis treated?
Fenbendazole or macrocyclic lactones
Where is angiostrongylus vasorum located geographically?
- Canada
- Europe
*“French heart worm”