* Respiration (Ch 21) Flashcards

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1
Q

Burning vs respiration

A

Similarities
- Oxygen is needed in burning and aerobic respiration, anaerobic respiration can take place in absence of oxygen
- Both produces carbon dioxide and water
- Both releases energy

Differences
Burning:
- a single step
- occurs everywhere
- no need enzymes
- energy released rapidly and uncontrolledly
- energy released in forms of light and heat

Respiration
- a series of chemical reactions
- occurs in living cells only
- need many enzymes
- energy released slowly, gradually in stepwise manner
- energy released in forms of ATP and heat

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2
Q

Mitochondria

A

Cristae membranes
- folding of inner membrane forms cristae
- membranous features increase surface area for holding electron transport chains and enzymes for ATP synthesis within limited amount of space

Circular DNA
- shows evidence of prokaryotic origin
- contains genes that code for some enzymes needed by mitochondria for energy transformation process

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3
Q

Cells having a lot of mitochondria to support their activities

A
  • liver cells: provide large amount of energy to support numerous metabolic activities
  • epithelial cells of small intestine: support active absorption of digested food
  • rod cells of retina: provide energy to support regeneration of visual pigment
  • sperm: supply energy for sperm to swim towards the egg
  • muscle fibres: provide large amount of energy to support muscle contraction
  • nerve fibres: resynthesis of neurotransmitter
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4
Q

Glycolysis

A
  • In cytoplasm
  • Glucose is stable that has to be activated by adding 2 ATP
  • Glucose splits into two molecules of triose phosphate
  • Each triose phosphate is oxidized to a pyruvate by losing hydrogen atoms
  • The hydrogen atoms are accepted by hydrogen carrier NAD to from NADH
  • Energy released in each oxidation process is used to form 2 ATP from 2 ADP and 2 phosphate groups
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5
Q

Krebs cycle

A
  • In matrix of mitochondria

Link reaction
- Pyruvate loses a molecule of carbon dioxide, loses hydrogen atoms which are accepted by NAD to form NADH
- The remaining acetyl group temporarily combines with coenzyme A to from acetyl coenzyme A

Krebs cycle
- Acetyl-coA combines with 4-C compound to form a 6-C compound, coenzyme A is regenerated to accept other acetyl groups
- 6-C compound is oxidized in a step wise manner to regenerate 4-C compound which allows the cycle to sustain
- 2 molecules of carbon dioxide are released
- 4 pairs of hydrogen atoms are accepted by 3 molecules of NAD and 1 molecule of FAD to from 3 NADH and 1 FADH respectively
- 1 ATP is directly produced

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6
Q

Oxidative phosphorylation

A
  • In inner membrane of mitochondrion
  • When the electrons carried by NADH and FADH pass along the carriers of electron transport chain, a series of redox reactions occur
  • The electron transport chain involves oxidation in removal of hydrogen atom and phosphorylation in formation of phosphate bonds in ATP
  • Electrons are passed downhill to oxygen, energy released is used to form ATP from ADP and P
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7
Q

Role of oxygen in respiration

A
  • The electrons and hydrogen atoms are accepted by oxygen to form water
  • Oxygen is the final hydrogen acceptor, which accepts hydrogen atoms from NADH and FADH, regenerated NAD and FAD, which can be used as hydrogen carriers in glycolysis and Krebs cycle, sustaining glycolysis and Krebs cycle
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8
Q

Alcoholic fermentation

A
  • Carried by yeasts and plants
  • In absence of oxygen, pyruvate is reduced by NADH to form ethanol and carbon dioxide
  • Pyruvate loses a molecule of carbon dioxide to form ethanal, which acts as final hydrogen acceptor to receive hydrogen atoms from NADH formed in glycolysis, regenrates NAD, glycolysis can sustain and produce ATP
  • Product is ethanol
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9
Q

Lactic acid fermentation

A
  • Pyruvate acts as final hydrogen acceptor that receives the hydrogen atoms from NADH formed in glycolysis, regenrates NAD, glycolysis can sustain to produce ATP
  • Product is lactic acid
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10
Q

Lactic acid fermentation in skeletal muscle in human

A
  • During vigorous exercises, though the human body increases rate and depth of breathing, oxygen delivered to muscles is still insufficient to support aerobic respiration fast enough to produce enough energy aerobically to meet all energy needed
  • Skeletal muscles carry out anaerobic respiration to provide extra amount of energy within a short period of time to support more vigorous muscular contraction
  • Lactic acid formed accumulates, causing muscle fatigue, muscles feel tired and fail to contract any more
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11
Q

Repaying oxygen debt

A
  • After exercise, the rate and depth of breathing is still higher than normal
  • Extra oxygen is taken in to break down lactic acid to provide additional amount of energy to convert lactic acid to glycogen for storage in liver
  • Restore blood pH to normal
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12
Q

Aerobic respiration vs anaerobic respiration

A

Similarities
- Fate of pyruvate molecules generated in glycolysis
- Oxygen requirement
- End products
- Energy yield

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13
Q

Aerobic respiration vs alcohol fermentation

A
  • Similarities
  • Products
  • Final hydrogen acceptor
  • Krebs cycle
  • Electron transport chain
  • Oxidative phosphorylation
  • Completion of oxidation of sugar
  • Energy production
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14
Q

Interrelationship between photosynthesis and respiration

A

Flow of energy:
- During photosynthesis, producers captures solar energy and converts it to chemical energy, which is stored in form of complex organic matter
- Chemical energy is passed to consumers through feeding
- In respiration, organic compounds formed in photosynthesis will be broken down by oxidation to release energy in form of ATP to support energy-requiring activities

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15
Q

Compare and contrast: photosynthesis and respiration

A
  • Energy transformation
  • Reactions involved in energy transformation: activation process and products formed, electron transport system and ATP synthesis, further transformation of energy from ATP and NADPH in photosynthesis
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16
Q

Heat production investigation

A
  • Thermo flask: prevent entry and escape of heat
  • Flask no completely filled with seeds: some air is left for respiration of seeds
  • Plug mouth of thermo flasks with cotton wool: allow diffusion of gases in or out more easily
  • Invert thermo flask: heat produced by the seeds warms the air, warm air is lighter so it rises up and in trapped in inverted flasks / carbon dioxide produced by the seeds is denser than air, escapes easily out of the inverted flasks / thermometer can be read easily
  • Soak the seeds in water before experiment: initiate seed germination
17
Q

Differential air thermometer

A
  • Insulating layer: prevent heat loss from chambers
  • Filter paper: ensures maximum surface area of potassium hydroxide solution available to contents of the chamber
18
Q

Showing release of carbon dioxide during respiration of plants

A
  • Light-proof cover: prevent photosynthesis in plant
  • Carbon dioxide-proof bag wrapping the pot: prevents carbon dioxide from soil organisms from escaping
19
Q

Explanation: movement of water drop

A
  • The plant absorbs O2 and release CO2 during respiration
  • CO2 released are absorbed by soda lime / potassium hydroxide / sodium hydroxide
  • Air pressure inside the test tube becomes lower than atmospheric pressure
  • Liquid position moves inwards to test tube

In control (environmental factors):
- Outwards: temperature increase / atmospheric pressure decrease

20
Q

Experiment of alcoholic fermentation

A
  • 10% glucose solution: more concentrated glucose solution has lower water potential the cell sap of yeast -> yeast cells lose water by osmosis and die
  • Boil glucose solution: drive off dissolved oxygen / kills microorganisms in solution (respiration affect results)
  • Cool boiled mixture before mixing: prevent yeast cells from being killed
  • Paraffin oil: prevent oxygen in air from dissolving into solution -> provide anaerobic condition
  • At the end of experiment: glucose is used up and ethanol builds up kill the yeast
21
Q

Production of biogas

A

Advantages:
- Reduces pollution
- Reduces consumption of fossil fuels
- Reduces use of chemical fertilizers
- Wastes can be recycled into useful matter

Limitations:
- Land is too expensive
- Smelly
- Problems in collection and transport of wastes
- Energy production cannot meet the great demand