Respiration Flashcards
how can ATP prod be affected by mitochondrial disease?
- affect proteins involved in OP and krebs cycle
- may cause anaerobic resp to inc
- = lots lactate —> muscle fatigue & weakness
- some lactate DIFFUSES into bloodstream —> high lactate conc in blood
what other respiratory substrates can be used in aerobic resp?
- fatty acids
- proteins
what does the rate of CO2 production indicate?
respiration rate
give one way to measure CO2 production?
use gas syringe
aerobic resp experiment?
- put known vol and conc of substrate sol (glucose) in test tube
- add known vol of buffer sol - keep pH constant
- place test tube in water bath set to one of temps
- leave for 10 mins to allow temp of substrate to stabilise
- add known mass of dried yeast to test tube and STIR for 2 mins
- after yeast dissolved - put bung with tube attached to gas syringe in top
- gas syringe set to 0
- start stop watch as soon as bung on
- as yeast respire, CO2 formed travels up tube into gas syringe
- at reg intervals, record vol of CO2 present in gas syringe
- do this for set amount time e.g. 10 mins
- repeat at each temp - calc mean
anaerobic resp experiment?
- same apparatus as aerobic resp experiment
- but after yeast dissolved - add liquid paraffin down inside test tube so SETTLES
- completely covers surface of sol
- stop oxygen getting in –> anaerobic resp
- put bung with tube attached to gas syringe in top
- gas syringe set to 0
- repeat rest of steps from aerobic resp experiment
what can respirometers indicate?
rate of aerobic respiration by measuring amount of oxygen consumed by organism over period of time
respirometer experiment
- apparatus set up so partially submerged in water bath at 15 degrees = optimum temp for woodlice and enzymes
- control tube set up - woodlice substituted for glass beads of same mass
- for 10 mins tap left open, syringe removed - allow apparatus equilibriate and resp rate of woodlice stablise
- syringe used reset monometer - so ends of fluid are at same level on either side of U
- reading from vol scale recorded
- as resp occurs, vol in air in test tube with woodlice dec - due to oxygen consumed
- CO2 absorbed by KOH
- decrease in vol of air = dec in pressure = coloured fluid in capillary tube of manometer to move towards it
how can oxygen consumption also be calc?
recording movement of fluid in manometer
- allow respiration rate of seeds to stabilise
- allow equilibrium to be reached
- allow for expansion / pressure change in apparatus
- optimum for enzymes in aerobic resp
- optimum for normal growth of seeds
- pressure dec in B due to higher vol of O2 uptaken BY SEEDS
- CO2 given off absorbed by KOH sol
suggest one reason why it was important that the student left the apparatus for one hour after the yeast culture reached a constant temp?
- so oxygen used
during the investigation, why did the liquid move right?
- anaerobic respiration produced CO2
- inc in pressure of gas
why is a log scale used to record the number of cells?
large range of bacteria
