Photosynthesis Flashcards
substance X enters mitochondrion from cytoplasm.
each molecule of substance X has 3 carbon atoms.
name substance X?
pyruvate
in the link reaction, pyruvate is converted to a substance with molecules effectively containing only 2 carbon atoms.
describe what happens in this process?
- acetylcoA formed
- Co2 formed
- NADH formed
describe the role of coenzymes and carrier proteins in the synthesis of ATP?
- NAD reduced
- electrons transferred from co-enzyme to co-enzyme
- energy used to synthesise ATP from ADP and phosphate
you can measure the rate of dehydrogenase activity by measuring what?
rate at which DCPIP loses blue colour
if dehydrogenase activity is taking place, what will happen?
- abosrbance dec as DCPIP gets reduced
- loses blue colour
- faster absorbance dec
- faster rate of dehydrogenase activity
you should also check whether the absorbance changes at each distance in 2 negative control tubes.
how can you do this?
- first tube contains ONLY DCPIP and chilled isolation sol
- second contains DCPIP and chloroplast extract - wrapped in tin foil (no light reaches)
NO CHANGE IN ASBORBANCE SHOULD BE SEEN BETWEEN THESE TWO
the solution that the student used to produce the chloroplast suspension had the same water potential as the chloroplasts.
explain why it was important that these water potentials were the same?
- no osmosis
- prevents chloroplasts damaging / bursting
explain why the student set up tube 1?
- to show light doesnt affect DCPIP
- to show chloroplasts needed
explain the results in tube 3?
- DCPIP reduced by electrons
- in chlorophyll
allows comparison between weed killers
- less ATP produced
- less NADPH produced
explain why the scientists measured the rate of production of oxygen in this investigation?
- oxygen produced in LDR
- faster oxygen produced, faster LDR
the scientists suggested that mutant plants producing more chlorophyll b would grow faster than normal plants in all light intensities.
explain how these data support this suggestion?
- faster LDR
- more sugars produced
- more energy for respiration and growth
- faster production of ATP and NADPH
heat stress decreases LDR of photosynthesis.
explain why this leads to a decrease in LDR?
- less ATP produced
- less NADPH produced
the experiments were carried out at high carbon dioxide concentration.
why?
so CO2 not limiting factor
explain why it was important to grow the plants under the same conditions up to ten days before the experiment
to make sure ONLY iron-deficiency causing difference
the plants were left in the dark for 6 hours before the experiment
why?
so same amount of triose phosphate at start