respiration

Question 1

respirometer

Answer 1

can be used to measure oxygen uptake of living organisms.soda lime is used to abosrb co2 produced.Use a syringe to set manometer fluid level and leave for 20 mins before measuring distance moved by fluid.set up a control tube containing same mass of beads .Caluclate volume of oxygen uptake by doing distance times 2pir

Question 2

anaerobic repsiration in mammals

Answer 2

pyruvate gains hydrogen from reduced nad forming lactate catalysed by lactase dehydrogenase

Question 3

where does chemisomosis occur

Answer 3

inner mitochondiral membrane

Question 4

does respiration require aqueous medium

Answer 4

yes so enzymes and substrate can collide,In dry conditions movement is limited

Question 5

how do you respire proteins

Answer 5

protein hydrolyzed by protease enzymes so amino acids are produced. Amino acids enter Krebs cycle after deamination.Deamination doesn’t release hydrogens so the ratio of hydrogen and carbon is lower so less energy produced

Question 6

how do you add red fluid to capillary tube

Answer 6

dip into small beaker and allow to run

Question 7

substrate level phosphorylation

Answer 7

2 atp made from glycolysis when tp is converted to pyruvate.Net yield of 2 atp.1 ATP produced per turn of the krebs cycle

Question 8

why does aerobic respiration yield fewer atp than expected

Answer 8

some atp used to actively transport pyruvate to mitchondria
not all protons move back through atp synthase as inner mitochondrial membrane is leaky so atp not made
some energy released in etc is used released as heat