Required Practicals - Using a light Microscope Flashcards
1
Q
What is the point of this practical?
A
To investigate cells and see their size, what they look like and how they work
2
Q
How do you work out Magnification
A
Image size divided by actual object size
3
Q
What do you use to make the cell visible?
A
A coloured stain
4
Q
What do you need to do with the specimen?
A
Make it as flat and thin as possible
5
Q
How do you focus on the specimen?
A
Start on the smallest lens, focus then move up a lens.
6
Q
How do you measure the size?
A
Use a ruler or eyepiece scale
7
Q
1mm =?
A
1000um (micrometre)
8
Q
1um =?
A
1000nm (newton metre)
9
Q
Why can you see the nucleus and cell wall but not mitochondria?
A
They are far too small and not stained
10
Q
How can you identify smaller parts of cells?
A
Use an electron microscope which has better resolution and magnification
11
Q
Method:
A
- place tissue sample on the microscope slide
- add few drops of suitable stain
- Lower a coverslip onto the tissue
- place slide on the microscope slide and focus using low power
- change to high power and focus
- Draw types of cells that are observed
- Add scale line to diagram
