required practical - culturing microorganisms

Question 1

explain the process of completing a microorganism practical

Answer 1

1. clean workspace with disinfectant - to kill any microorganisms that could contaminate culture
2. sterilise a loop by passing it through a Bunsen burner flame
3. open a sterile agar plate near a flame - killing any bacteria in the air
4. use the loop to spread chosen bacteria evenly over the plate
5. place sterile filter paper disks containing different antibiotics onto the plate
6. incubate the plate at 25 degrees Celsius

Question 2

how can you measure the effect of an antibiotic

Answer 2

calculate the area of the zone of inhabitation
= pi x r squared

Question 3

what indicates the best antibiotic

Answer 3

if the zone of inhabitation is bigger

Question 4

why do you incubate at 25 degrees

Answer 4

reduces the chanced of harmful bacteria growing