Reproduction Flashcards
Steroids
Made from cholesterol in adrenal cortex, testis, ovary, placenta
Mode of action:
- Freely enter target cells
- Interacts with specific receptor molecules inside cell
- Receptor hormone complex -> nucleus
Progestagens
Prepare uterus to receive embryo and maintains uterus during pregnancy
Stimulates mammary gland growth (suppresses milk secretion)
Regulates secretions of gonadotrophins (FSH, LH) produced by pituitary gland
Androgens
Differentiation of male tissue in embryo and secondary sexual characteristics
Supports spermatogenesis
Influences aggressive and sexual behaviour
Regulates secretions of gonadotrophins (FSH, LH)
Oestrogens
Secondary sexual characteristics
Prepare uterus for sperm transport
Stimulate growth and activity of mammary gland, gonads and endometrium
Regulates of gonadotrophins (FSH, LH)
Eicosanoids
Derived from arachidonic acid (AA) Local hormone (half life 10 mins) Leukotrienes and prostaglandins
Prostaglandins
Erection and ejaculation Luteal regression Ovulation Uterine contraction Uterine contraction Cervical softening Milk ejection
Protein and peptide hormones
Mode of action:
- Via plasma membrane receptors binding to receptors generate secondary messengers
- Activation of kinases
- Phosphorylates proteins -> physiological action
Glycoproteins: FSH, LH
Polypeptide: ACTH, prolactin (PRL)
Peptides: Gonadotrophin-releasing hormone (GnRH), oxytocin (OT)
Hypothalamic
Small part of brain - behavioural and stress responses
Neuroendocrine centre
Many pass to pituitary gland for processing
Cortico-release hormone (CRH)
Stimulates ACTH secretion (ACTH -> adrenal gland -> cortisol)
Growth hormone releasing hormone (GHRH)
Stimulates growth hormone release
Dopamine (DA)
Inhibit prolactin release
Gonadotrophin releasing hormone (GnRH)
Stimulates LH and FSH release
Half life 7 mins
Pulsatile secretion
- Neural control -> pulsatile release
- Non-neural control -> more sustained release
Synthesised by nerve cell bodies in hypothalamus -> arterial pituitary via blood -> anterior pituitary secrete LH, FSH
FSH (follicle stimulating hormone)
Growth and maturation of ovarian follicle
Acts mainly on granulosa (surrounds oocyte) cells of follicle
Artificially stimulate multiple ovulation for IVF
Affects spermatogenesis in testis - acts on Sertoli cells
Stimulates formation of oestradiol in ovary and testis (with LH)
Luteinising hormone
Pulsatile secretion - frequency and amplitude vary - control ovarian cycle
Pre-ovulation surge of LH -> follicle rupture and ovulation
Regulates progesterone synthesis after ovulation in CL
Stimulates formation of oestradiol in ovary with FSH
Regulate testosterone synthesis in testis - Leydig cells
Oxytocin-uterine contraction
Afferent sensory nerve impulse -> spinal cord -> midbrain -> produced in hypothalamus -> posterior pituitary -> myometrium to contract
Oxytocin - milk ejection reflex
Neural hormonal reflex
Afferent sensory nerve impulses -> spinal cord -> midbrain -> hypothalamus -> neurosecretory cells to discharge oxytocin form axons end in posterior pituitary -> bloodstream -> stimulates myoepithelial in ducts of lactating mammary glands to contract
Milk from alveoli to ducts to nipple to infant
Stimulate increased prolactin secretion - duct development and milk synthesis
Sertoli cells
Inside seminiferous tubules
Spermatocytes receive testicular proteins via Sertoli cell gap junction
Spermatocytes and spermatids physically attached to Sertoli cells
Removes material from elongating spermatid during cytoplasmic condensation - removal of excess cytoplasm
All linked by gap junctions - communication throughout tubule
Mediate actions of hormones on spermatogenesis
Spermatogenesis
Mitotic proliferation:
- Large numbers of spermatogonia (diploid) produced
- Basal compartment of tubule
Meiotic division:
- Spermatocytes -> spermatids
- Adluminal compartment of tubule
Cytodifferentiation:
- Packages genes for delivery to oocyte
- Elongating spermatid -> spermatozoa
Testosterone
Synthesised by Leydig cells - between seminiferous tubules
Passes into blood and seminiferous tubules
Sertoli cells convert testosterone -> dihydrotestosterone - passes into testicular fluid - stimulate male reproductive tract
LH stimulate Leydig cells to make testosterone
FSH stimulates production of androgen receptor proteins in Sertoli cells
Epipdidymal maturation
Sperm structure: loss of surplus cytoplasm, condenstaion of nuclear chromatin
Sperm membranes: surface glycoproteins added, membrane fluidity and lipid compisition change
Metabolism: Depressed - prolong life, increased dependence on external fructose
Motility: increase cAMP content of tail
Seminal plasma composition
(From prostrate, seminal vesicles, ampulla)
Glycoproteins - decapacitation factors
Fructose and sorbital - energy
Citric acid - stops cell coagulation
Acid phosphatase - phospholipid metabolism
Buffers
Ascorbic acid - protect sperm from oxidation
Prostaglandins - muscle contraction in female tract
Penis shaft
Corpus cavernosum - main body
Corpus spongiosum - urethra
Blood reservoirs during erection
Fibroelastic penis
Bull, boar, ram
Limited erectile tissue, sigmoid flexure
Sigmoid flexure
Retract penis inside body until erection
Help by retractor penis muscle
Contract - held in
Relax - protrusion
Musculovascualr penis
Stallion
Large corpus cavernosum - fills with blood
No sigmoid flexure
Retractor penis muscle
Erection and ejaculation
Stimulation of pelvic nerve, arterial dilation, increased blood flow to corpus cavernosum
Retractor penis muscle relaxes (sigmoid flexure straightens)
Muscles of vas deferens, smeinal vesicles and prostrate contract
Spermatozoa and seminal plasma expelled
May be fractions
Follicles
Females born with fixed number of primordial follicles
Contains granulosa and theca - hormone producing
And gamete - oocyte
Folliculogenesis
Primordial follicle:
- 1 primary oocyte, 1 layer of pre-granulosa
Primary follicle:
- Increased oocyte size
- 1 layer of cuboidal granulosa cells
- Zona pellucida separates oocyte from granulosa cells
Secondary follicle:
- Multiple layers of granulosa cells - oocytes fully grown
- Theca - interna, externa
- Vascularisation of theca layer
- Theca calls produce antigens - substrate for oestrogen synthesis (granulosa cells)
Tetiary (antral) follicle:
- Granulosa secrete fluid
- Antrum formed (fluid filled)
- Theca internal cells become steroidogenic
Follicle maturation
Antral follicles reposnd to FSH Dominant follicle selected - Graafian follicle - Dependent on LH - Granulosa cells acquire LH receptors - Ovulation caused by LH surge
Corpus luteum
After ovulation, follicle collapses and remaining granulosa and luteal cells form the corpus luteum
Numbers of CL - number of ovulations and release oocytes
Remains for duration of luteal phases, rapidly invaded by blood vessels
Synthesise progesterone to maintain pregnancy
Formation regulated by LH
Seasonal breeders
Sheep, goat, horse, deer
+/- cat
Wild pig
Hamster, rabbit
Non-seasonal breeders
Dog
Cow, pig
Guinea pig
Rodents
Physiological basis of seasonal breeding
Neuroendocrine pathway
Light -> pineal gland produces melatonin -> hypothalamus produces GnRH -> pituitary produces LH pulses -> oestrous cycle -> ovulation of oocytes
Sperm motility
Moving through uterine tract: uterine cilia, uterine contractions
Sperm tail: energy production, propulsive apparetus
Axenome: similar to flagella
Sperm structure
Tail:
- Mitochondrial helix - energy
- Axenome - 2 central microtubules surrounded by 9 microtubule doublets
Head: Apical ridge, acrosome, plasma membrane, equatorial segment, almost no cytoplasm
Sperm transport
Rapid transport phase:
- Reaches oviducts within minutes
- No time to mature, unable to fertilise
Sustained transport phase:
- Capacitation and hyperactivation in female tract
- Storage reservoirs - cervix and oviducts
Sperm storage
Extends window for fertilisation
Cervical storage: cervical crypts - blind ending tunnels get rid of bad swimming sperm, absence of progesterone - sperm released
Oviduct sperm storage: uncapacitated sperm bind to epithelial cells in the isthmus, at ovulation number of sperm in the oviduct increase
Fertilisation
Acrosomal enzyme -> small hole in zona pellucida (rapid process)
Sperm move into perivitelline space between zona pellucida and oocyte plasma membrane
Oocyte plasma membrane fuses with sperm equatorial segment - sperm engulfed
Cortical granules from oocyte move into perivitelline space to cause zona pellucida to block polyspermy
Pregnancy - progesterone
Corpus luteum:
- Maintains progesterone production
- 2 weeks in non fertile cycle (lysed by PGF2a)
- Continues if pregnant (until placenta takes over- primates, horses)
- Implanting foetus must signal its presence to prevent removal of progesterone
PMSG
Pregnant mare serum gonadotrophin
AKA equine chorionic gonadotrophin - luteotrophics
Secreted by trophoblasts cells on day 40-120
LH-like activity - promotes follicular growth, ovulation and production/maintenance of CL
Placenta takes over from CL around day 140
Maternal support of pregnancy
Adequate metabolism of O2, salts and organic pre-cursors
Conceptus induces formation of placenta
Development and hypertrophy of the uterine musculature
Development and maintenance of mammary glands
Take over motherās metabolism with pregnancy hormones (progesterone, oestrogen, CG, placental lactogen, PL)
Labour - stage 1
Regular uterine contractions Cervical mucous plug Cervical shortening and dilation occurs Latent phase: cervix slowly dilates to 3cm Active phase: rapid dilation of cervix
Labour - stage 2
Complete delivery of foetus
Rupture of membrane and abdominal contractions
Labour - stage 3
Delivery of placenta
dogs and cats: occurs with stage 2
Myometrial contractions
Co-ordinated uterine contraction requires the simultaneous activation of all smooth muscle cells in the uterus
Brachystasis
Contractions lead to retraction of the lower uterine segment and cervix upwards
Creates a birth canal
Myometrial cells undergo brachystasis - muscles contract and shorten but do not regain their original length at relaxation
Contraction hormones
Oxytocin: lowers the excitation threshold of muscle cells
Prostaglandins: stimulate liberation of Ca2+ from intracellular stores
Cervical softening
High connective tissue content Resist stretch, allowing distension of the body of the uterus whilst maintaining the cervix in a closed state Softening - 2 changes: - Reduction of collagen fibres - Increase in proteoglycan matrix fibres
Regulated by prostglandins
White vaginal discharge
Vaginitis
Early metoestrous
Open pyometra
Cystitis
Red vaginal discharge
Proestrous Oestrous Persistant ovarian follicle Ovarian tumour (oestrogen secreting) Vaginal trauma/FB Cystitis Urethral neoplasia Coagulopathy Placental separation Subinvolution Post partum Vascular malformation
Vaginal discharge - other colours
Clear: normal Clear watery: amnotic/allontic fluid Greeny/black: normal parturition/dystocia Brown/red-black: metritis Yellow: incontinence
Vaginitis
Usually purulent discharge in a bitch
Age at onset important to determine:
- Juvenile: secondary to bacterial contamination and excess vaginal secretion, usually resolves spontaneously with 1st season, avoid Abs
- Adult: less common, ID and treat specific causes, may respond to exogenous oestrogens
Pyometra
Uterus filled with pus - usually within 8 weeks of last oestrous
Open: mucopurulent vaginal discharge and mild-moderataly enlarged uterus
Closed: no discharge, grossly enlarged uterus, systemic illness
Depression, lethargy, mucopurulent discharge, pyrexia, PU/PD, V, collapse, shock, neutrophils with a left shift, possible azotaemia, acidosis, endotoxaemia, hypoglycaemia, anaemia, coagulation abnormalities
Vaginal/vestibulo neoplasia
Smooth muscle tumour of the vagina/vestibule - most common
Typically slow growing smooth muscle tumours
- Leiomyoma, leiomyosarcoma
- Usually elderly entire bitches
- May present with visible mass, bulging perineum or dysuria/dyschezia
Treat: surgical excision and spay, chemotherapy?
Vaginal hyperplasia and vaginal prolapse
Excessive response of vaginal mucosa to oestrogen during follicular phase of oestrous cycle - vaginal oedema/prolapse
Brachycephalic breeds are predisposed, may interefer with mating, exposed tissues may get traumatised
Treat: Conservative measures - moist, vulvar sutures, surgical excision, usually via episiotomy, spay/control oestrous
Ambiguous genitalia
Indication of presence of androgens and therefore testicular material +/- ovarian tissue
Investigate: evaluate pelvic anatomy, remove gonads + histopath, karotyping
Treat: remove gonads, possible partial penile amputation possible via an episiotomy
Recognise dystocia in the bitch (and queen)
Foetal fluid passed more than 2-3h but no birth
Vigorous, regular straining for 20-30min but no birth
Greenish/reddish brown vulval discharge apparent but no birth
>2-4h since last pup/kitten (def more coming)
Second stage of labour >12h
Sickness of dam
Maternal causes of dystocia
Narrow birth canal Disturbed labour: uterine inertia (most common), uterine spasm/tetany, inadequate abdominal forces Uterine abnormalities Prolonged pregnancy Pyschogenic status Extra uterine problems Premature birth Prolonged parturition Idiopathic
Foetal causes of dystocia
Increased foetal size/litter size, gestational length, genetic/breed factors
Foetal malpresentation (most common)
Abnormal foetal development - hydrocephalus, other congenital abnormalities, foetal death
Uterine inertia
Primary: more common, uterus fails to response to foetal signs, complete or partial
Secondary: exhaustion of the myometrium, secondary to an obstruction
Medical management of dystocia
Dog/Cat
No evidence of obstruction
Exercise dam, feathering the roof of vaginal floor, treat hypocalcaemia/hypoglycaemia, tocospasmolytic drugs sometimes
Oxytocin:
Repeat small doses - 0.2-0.4 IU/kg every 30-40 min
Then consider caesarean
Vulval/vaginal abnormalities
Congenital: vulval stenosis, anovulvar cleft, rectovaginal fistula, vestibulovaginal stricture/band
Acquired: Vulval hypertrophy, recessed vulva, trauma, neoplasia
Ovarian neoplasia
Relatively uncommon in cats/dogs Granulosa cell tumour Cystadenoma Adenocarcinoma Teratoma
Large mass +/- ascites
May be endocrinologicall active
Surgical excision
Hydrometria
Sterile accumulations of fluid within uterus
Large fluid filled viscous within abdomen
Rare, may be incidental
Cryptorchidism
Most common congenital defect in male dog (occasionally cat)
May be abdominal/inguinal/prescrotal
More common in pedigrees
More susceptible to torsion and neoplasia - castrate
Anorchism/monorchism very rare
Testicular neoplasia
2nd most common site in male dogs (rare in cats)
Three types of roughly equal incidence:
- Seminoma (may rarely be assocated with feminisation)
- Interstitial cell tumour aka Leydog tumours (functional - produce testosterone)
- Sertoli cell tumour (functioning produce oestrogen)
Usually benign if scrotal (not retained)
Can cause infertility
Orchitis/epidiymitis
Usually together
Signs: epididymal enlargement, testicular pain, tenesmus and scrotal oedema, may abscessate via scrotum, systemic illness
Chronic: small, firm testes with epididymal enlargement, adhesions between tunics and scrotum may reduce testicular motility
Infection may originate from urinary tract, via direct penetrations or via haematogenous spread
Castrate
Protruding penis
Social problem for owner, low grade irritation, pain, bleeding, trauma
Paraphimosis: Non-erect penis protrudes from prepuce and cannot be retracted or retained in its normal position - narrowed preputial orifice, penile enlargment
Priapism, trauma
Failure of penis to stay in prepuce:
- Abnormally short prepuce
- Weak preputal muscles
- Wear retractor penis muscle
- Contracture following wound
Treat: Symptomatic, surgical enlargement of preputial opening, phalloplexy, preputial lengthening/reconstructive procedures, partial penile amputation