Recombinant DNA Flashcards
Restriction Endonucleases
Enzymes that cut the DNA backbone at a particular sequence of bases
Restriction Site
A particular sequence of DNA that a restriction enzyme recognizes and cuts
Plasmid
Small, circular, double-stranded DNA molecule that is separate from the cellular chromosomal DNA and that contains its own origin of replication; found in bacteria, yeast, and viruses
Horizontal Transfer
The movement of genetic information from one organism to a different organism that is not a progeny of the first organism
Cloning Vector
A plasmid with multiple restriction sites, an origin of replication, and a gene encoding a protein allowing for selection
Recombinant DNA
DNA from two or more sources formed into a single molecule as a result of cloning or recombinant DNA technology
Complementary DNA (cDNA)
DNA that is complementary to a segment of ssRNA, typically do not contain introns when complementary to mRNA
Recombinant Genomic DNA Library
A collection of the total genomic DNA from a single organism stored within identical cloning vectors
Polymerase Chain Reaction (PCR)
A laboratory technique used to exponentially amplify a segment of DNA using DNA primers and a heat-resistant DNA polymerase for replication
Gel Electrophoresis
Laboratory method where electricity is passed through a porous gel matrix to separate mixtures of either nucleic acids or proteins by molecular size
Molecular Weight Ladder
A mixture of DNA segments of known lengths used as a reference in gel electrophoresis of nucleic acids
Vector
vector, a molecule that can somehow replicate–
and here’s somehow the instructions to allow this piece of DNA to be copied
Why Bacteria have restriction enzyme?
A defense mechanism; to cut viral DNA
Methylase
An enzyme add a Methyl group (CH3)
what binds to a promotor
RNA polymerase
