rationale Flashcards

1
Q

identify the type of light:

shortest wavelength but highest energy

A

ultraviolet

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2
Q

identify the type of light:

longest wavelength but lowest energy

A

infrared

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3
Q

range of visible light

A

400-700nm

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4
Q

identify the wavelength used

caraway method for uric acid

A

650-700nm

(+) tungsten blue - visible light

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5
Q

identify the wavelength used

uricase method for uric acid

enzymatic method

A

293nm

ultraviolet

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6
Q

identify the wavelength used

dubowski reaction for glucose

orthotoluidine/condensation method

A

620-730nm

(+) bluish green - visible light

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7
Q

identify the wavelength used

LDH method

A

340nm

ultraviolet light

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8
Q

identify the wavelength used

cyanmethemoglobin method

A

540nm

visible light

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9
Q

identify the wavelength used

bilirubin in amniotic fluid

A

OD450nm

visible light (test for HDN)

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10
Q

In nephelometry, what scatter measures size/volume?

A

forward scatter

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11
Q

In nephelometry, what scatter measures internal cell complexity?

granularity and lobularity

A

side scatter

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12
Q

measures blocked light

A

turbidimetry

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13
Q

light source for visible-IR light range

A

tungsten-halogen (iodide) lamp

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14
Q

an aspect of QA that is used to assess analytical phase of patient testing

A

quality control

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15
Q

anticoagulant preferred for erythrocyte osmotic fragility and electrolytes testing

A

heparin

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16
Q

color of citrate anticoagulant cap for ESR testing

A

black

3.8% anticoagulant (1:4)

17
Q

photolabile analytes

A

bilirubin and CK

18
Q

cold-labile analytes

A

LD4 and LD5

stored at RT

19
Q

an indicator of precision

A

standard deviation

20
Q

relative indicator of precision

A

coefficient of variation

21
Q

test to evaluate accuracy

22
Q

test to evaluate precision

23
Q

serum pool or pooled sera; material of known value that is analyzed with patient samples to determine acceptability of results

24
sudden or abrupt change in the data
shift
25
gradual deviation in the data
trend
26
Fluorometers are designed so that the path of the exciting light is at the right angle to the path of the emitted light. What is the purpose of this design?
prevent loss of emitted light
27
Lamp that may be described as containing **argon** and having the element of interest coated on its cathode?
tungsten lamp
28
In addition to utilizing Levey Jennings charts, what other criteria should be applied to interpret internal quality control data?
westgard multirule
29
refers to the measure of **scatter** of experimental data around the mean of a Gaussian (normal) distribution curve?
standard deviation
30
What term describes the extent of agreement between **repeated analyses**?
precision
31
refers to the deviation from the true value caused by **indeterminate errors** inherent in every laboratory measurement?
random error
32
closeness with which the **measured value agrees with the true value**
accuracy
33
describes the ability of an analytical method to **maintain both accuracy and precision** over an extended period of time?
reliability
34