[LB] 01. Laboratory Safety + Pipetting Flashcards by Christianne Espiña

Q

What administration is authorized to conduct on-site inspections to determine whether an employer is complying with the mandatory standards?

A

Occupational Safety and Health Administration (OSHA)

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Q

Identify the standard.

Applies to all exposure to blood or other potentially infectious materials in any occupational setting.

A

Bloodborne Pathogens

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Q

Identify the standard.

Mandates the development of an exposure control plan.

A

Bloodborne Pathogens

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Q

Formerly referred to as universal precautions, this concept is basically an approach to infection control in which all human blood, tissue, and most fluids are handled as if known to be infectious for ALL bloodborne pathogens.

A

Standard Precautions

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Q

Who developed the standard hazard identification system (diamond-shaped, color-coded symbol)?

A

National Fire Protection Association (NFPA)

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Q

Identify the hazard based on NFPA’s system.

Blue quadrant.

A

Health hazard

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Q

Identify the hazard based on NFPA’s system.

Red quadrant.

A

Flammable hazard

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Q

Identify the hazard based on NFPA’s system.

Yellow quadrant.

A

Reactivity/Stability hazard

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Q

Identify the hazard based on NFPA’s system.

White quadrant.

A

Special hazards

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Q

What is the major source of safety information for employees who may use hazardous materials in their occupations?

A

Safety Data Sheet

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Q

Identify the safety equipment.

Contain and expel noxious and hazardous fumes from chemical reagents.

A

Fume hoods

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Q

Identify the safety equipment.

Remove particles that may be harmful to the employee who is working with potentially infectious biologic specimens.

A

Biosafety cabinets

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Q

Identify the standard.

Provides laboratories with specific guidelines for handling hazardous chemicals?

A

OSHA laboratory standard

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Q

Identify the standard.

Require laboratories that use hazardous chemicals to have a written chemical hygiene plan.

A

OSHA laboratory standard

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Q

Identify the classification of hazards in chemicals.

Substances that affect cellular development in a fetus or embryo.

A

Teratogen

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Q

Identify the classification of hazards in chemicals.

Interfere with metabolic processes when ingested, inhaled, or absorbed through the skin.

A

Toxic

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Q

Identify the classification of hazards in chemicals.

Capable of causing cancer.

A

Carcinogenic

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Q

Identify the classification of hazards in chemicals.

Visible destruction of human tissue (skin and eyes) on contact.

A

Corrosive

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Q

Identify the classification of hazards in chemicals.

Injurious to the skin or eyes by direct contact or to the tissue of the respiratory and gastrointestinal tracts if inhaled or ingested.

A

Corrosive

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Q

Identify the classification of hazards in chemicals.

Induce genetic mutations.

A

Mutagen

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Q

What refers to the lowest temperature at which sufficient vapor is given off to form an ignitable mixture with air?

A

Flash point

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Q

Identify the flash point.

Flammable

A

<100 F

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Q

Identify the flash point.

Combustible

A

> /100F

Q

What refers to the chemical reaction that involves the rapid oxidation of combustible material or fuel with subsequent liberation of heat and light?

A

Fire

What refers to the minimum temperature at which self-sustained ignition will occur?

Flash point

What form of radiation is a concern when equipment often emits a variety of wavelengths of electromagnetic radiation that must be protected against through engineered shielding or the use of PPE?

Nonionizing radiation

Enumerate the essential elements that conduct fire.

1. Fuel 2. Heat (Ignition source) 3. Oxygen (Air)

What class of fire should be left to professional firefighters?

Class D

What components of a fire extinguisher should be used for Class C fires?

CO2, Halon, and Dry chemical

Identify the combustible material. Class A fire

Ordinary combustibles (cloth, wood, paper, and fabric)

Identify the combustible material. Class B fire

Flammable liquids

Identify the combustible material. Class C fire

Electrical equipment

Identify the combustible material. Class D fire

Reactive metals

Identify the combustible material. Class E fire

Detonation

What components of a fire extinguisher should be used for Class D fires?

Metal X

What hazard involves liquid nitrogen?

Cryogenic material hazards

What hazard refers to the effect of repeated manipulation of instruments, etc.?

Ergonomic hazards

What hazard is involved when using equipment such as centrifuges, autoclaves, homogenizers, lab glasswares, glass pipets, etc.?

Mechanical hazard

Identify: A technique used in clinical chemistry involves transferring an aliquot of liquid sample from one container to another.

Pipetting

What are the classifications of pipettes according to design?

1. To Contain (TC) | 2. To Deliver (TD)

What are the classification of pipettes according to drainage?

1. Blowout | 2. Self-draining

Identify the pipette. Calibrated to accurately transfer the specified volume to another container.

TD pipette

Identify the pipette. Has markings that will accurately measure the volume to be contained.

TC pipette

Identify the pipette. The transferred liquid will not necessarily deliver the specified volume.

TC pipette

Identify the pipette. The last drop of pipette should be expelled into the receiving vessel.

Blowout pipette

Identify the pipette. Allow contents to be drained by gravity.

Self-draining pipette

What are the classifications of pipettes according to type?

1. transfer pipette | 2. measuring pipette

Identify the pipette. Can dispense a known volume of liquid.

Transfer pipette

Identify the pipette. Has graduation marks and can dispense a different volume of liquid.

Measuring pipette

Identify the pipette. A transfer pipette that is calibrated to accurately deliver a fixed volume of diluted aqueous solution.

Volumetric pipette

Identify the pipette. A transfer pipette that is used to accurately measure viscous fluids such as blood.

Ostwald-Folin pipette

Identify the pipette. A measuring pipette that is self-draining and has graduations above the tip.

Mohr pipette

Identify the pipette. A measuring pipette that is a blowout type and has graduations down the tip.

Serological pipette

Identify the pipette. A measuring pipette that can measure less than 1.0mL.

Micropipette

Identify the pipette. Pipetting devices that permit rapid repetitive measurement and delivery of precise volumes.

Automatic pipettes