[LB] 04. Electrophoresis

Question 1

What is the process by which charged molecules migrate through an electric field?

Answer 1

Electrophoresis

Question 2

Enumerate the components of an electrophoresis setup.

Answer 2

1. Power supply
2. Buffer
3. Supporting medium
4. Electrophoresis chamber

Question 3

What measures the density of light passing through the fraction?

Answer 3

Densitometer

Question 4

Identify the type of electrophoresis.

Generates a zone of heterogenous proteins that are physically separated from each other.

Answer 4

Zone electrophoresis

Question 5

Identify the type of electrophoresis.

Simultaneously separates several samples in a single run by using a rectangular thick gel.

Answer 5

Slab gel electrophoresis

Question 6

Identify the type of electrophoresis.

Uses agarose gel that yields five zones.

Answer 6

Disc electrophoresis

Question 7

Identify the type of electrophoresis.

Separates amphoteric compounds with increased resolution.

Answer 7

Isoelectric focusing

Question 8

Identify the type of electrophoresis.

Combines charge-dependent isoelectric.

Answer 8

Two-dimensional electrophoresis

Question 9

What type of electrophoresis provides the highest resolution for DNA fragmentation separation?

Answer 9

Two-dimensional electrophoresis

Question 10

Identify the type of electrophoresis.

Carried out in a small-bore, fused-silica capillary tube.

Answer 10

Capillary electrophoresis

Question 11

Identify the type of electrophoresis.

Separation of proteins by electrophoresis followed by an overlay of monospecific antibodies.

Answer 11

Immunofixation electrophoresis

Question 12

Identify the type of electrophoresis.

Employs a higher voltage in conjunction with a cooling system and a more concentrated buffer in the electrophoretic apparatus.

Answer 12

High-resolution electrophoresis