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ASCP M Mometrix > RAPID IDENTIFICATION AND ANTIBIOTIC RESISTANCE DETECTION OF MICROORGANISM > Flashcards

RAPID IDENTIFICATION AND ANTIBIOTIC RESISTANCE DETECTION OF MICROORGANISM Flashcards

1
Q

Where is S. aureus found?

A

In the nasal passages
is the most common to become pathogenic

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2
Q

What are the biochemical reactions of S. aureus?

A

Catalase positive
Coagulase positive

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3
Q

How does S. aureus appear on gram stain?

A

GPCs in “grape-like” clusters

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4
Q

What are the growth requirements for S.aureus?

A

aerobic and facultative anaerobe, grows= best at 35 °C in ambient
air or a CO2-rich environment

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5
Q

How does the colony morphology for S. aureus appear?

A

soft, opaque or pale gold, and circular on sheep blood agar, most
colonies are beta-hemolytic, and colonies present with a yellow halo on mannitol salt agar

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6
Q

Where is S. epidermidis found?

A

most prevalent skin flora, attach to the outer layer of skin,
the epidermis, and inhibit overgrowth of potential pathogens

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7
Q

what are the biochemical reactions of coagulase negative Staph?

A

Catalase positive
Coagulase negative

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8
Q

What are the growth requirements for coag negative staph?

A

aerobic and facultative anaerobe, grow best at 35 °C in ambient air
or a CO2-rich environment

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9
Q

What is the colony morphology of S. epidermidis?

A

white, cohesive, raised circular colonies on tryptic soy agar, opaque,
gray, smooth, raised colonies with no hemolysis on sheep blood agar

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10
Q

What is the colony morphology of S. saprophyticus?

A

white-yellow, opaque, smooth, raised colonies with a butter-like
texture on sheep blood agar.

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11
Q

What are the biochemical reactions of beta-hemolytic streptococci?

A

Catalase −
Gas production −
Motility −
Optochin R
Bile solubility −
Leucine aminopeptidase +
Vancomycin S

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12
Q

What does Streptococci look like on a gram stain?

A

GPCs in chains

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13
Q

What are the growth requirements of beta hemolytic streptococci?

A

5–10% CO2 with vertical stabs in agar to promote beta-hemolysis

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14
Q

What is the colony morphology of beta hemolytic streptococci Group A?

A

grayish-white, transparent to translucent, matte or glossy, large zone of hemolysis

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15
Q

What is the colony morphology of beta hemolytic streptococci Group B?

A

larger than group A colonies, translucent to opaque, flat, glossy, narrow
zone of beta-hemolysis, and some strains are nonhemolytic

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16
Q

What is the colony morphology of beta hemolytic streptococci Group C?

A

grayish-white, glistening, wide zone of beta-hemolysis

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17
Q

What is the colony morphology of beta hemolytic streptococci Group F?

A

grayish-white, small, matte, narrow zone of beta-hemolysis

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18
Q

What is the colony morphology of beta hemolytic streptococci Group G?

A

grayish-white, matte, wide zone of beta-hemolysis.

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19
Q

What are the biochemical reactions of Enterococcus species?

A

Catalase −
PYR +
Gas production +
Optochin R
Bile solubility −
Bile esculin +
6.5% NaCl +
Leucine aminopeptidase +

20
Q

what does the gram stain look like for enterococcus species?

A

GPCs in chains or pairs

21
Q

what are the growth requirements for enterococcus species?

A

aerobic and facultative anaerobe, able to grow in temperatures
ranging between 10 °C and 45 °C

22
Q

What is a “facultative” anaerobe?

A

an organism that can survive both with and without oxygen, preferring aerobic conditions when available

23
Q

What is an “obligate” anaerobe?

A

an organism that can only survive in the absence of oxygen and will die if exposed to it

24
Q

What is the colony morphology of enterococcus species?

A

large white colonies on sheep blood agar, may be alpha- or beta-
hemolytic, blackens medium of bile esculin agar during growth.

25
Q

how does the gram stain appear for Candida species?

A

Gram-positive single yeast buds, pseudohyphae constricted but connected at
the ends, or true septate hyphae

26
Q

What are the growth requirements for Candida species?

A

aerobic and facultative anaerobe, grow best at 25–37 °C

27
Q

What does the colony morphology look like in Candida species?

A

creamy, white, dull, grows upward with foot-like projections from the
bottom of the colony on sheep blood agar and cream-colored, smooth, pasty colonies on
Sabouraud dextrose agar.

28
Q

What are the biochemical reactions of streptococcus pneumoniae?

A

Catalase −
Bacitracin R
Na Hippurate −
Optochin S
Bile solubility +
Bile esculin −
6.5% NaCl −

29
Q

how does streptoccus pneumoniae appear on a gram stain?

A

gram positive diplococci, lancet shaped

30
Q

what is the colony morphology of streptococcus pneumoniae?

A

alpha-hemolytic, mucoid or “water drop” colonies with a convex
middle on sheep blood agar

31
Q

What are the biochemical reactions of ACINETOBACTER BAUMANNII?

A

Oxidase −
Growth on MacConkey agar +
Motile −
Glucose oxidation +
Maltose oxidation −
Esculin hydrolysis −
Lysine decarboxylase −
Nitrate reduction −
Urea V

32
Q

How does ACINETOBACTER BAUMANNII appear on gram stain?

A

Gram-positive/variable coccobacilli

33
Q

What are the growth requirements for Acinetobacter baumanni?

A

aerobic and facultative anaerobe, grow best at 35 °C in ambient air
or a CO2-rich environment

34
Q

What is the colony morphology of Acinetobacter baumanii?

A

smooth, creamy, opaque, raised on sheep blood agar, purplish hue on
MacConkey agar

35
Q

What are the biochemical reactions of Enterobacteriaceae?

A

Oxidase −
Catalase +
Nitrate reduction +

36
Q

what does the gram stain look like for enterobacteriaceae?

A

GNRs

37
Q

what are the growth requirements for enterobacteriaceae?

A

aerobic and facultative anaerobe, grow best at 35 °C in ambient air
or a CO2-rich environment. MacConkey and other selective agars are incubated in ambient
air only. Yersinia pestis grow best at 25–30 °C.

38
Q

what is the colony morphology of enterobacteriaceae?

A

large, smooth, and gray on sheep blood and chocolate agars.

39
Q

Lactose fermenting species, pink on MacConkey agar of enterobacteriaceae:

A

❖ Citrobacter
❖ Escherichia
❖ Enterobacter
❖ Klebsiella

40
Q

Non-Lactose fermenting species, pink on MacConkey agar of enterobacteriaceae:

A

❖ Shigella
❖ Yersinia
❖ Proteus
❖ Salmonella

41
Q

what are the biochemical reactions of pseudomonas species?

A

Motility +
Oxidase +
Indole +
Lysine decarboxylase −

42
Q

how does pseudomonas appear on gram stain?

A

GNRs

43
Q

what are the growth requirements for pseudomonas?

A

obligate aerobe, grow best at 35 °C in ambient air or a CO2-rich
environment

44
Q

what is the colony morphology of P. aeruginosa?

A

grayish white, translucent to opaque, circular with irregular edges on
sheep blood agar. Colorless, transparent, circular, on MacConkey agar. Colonies are
smooth or mucoid, if swarming occurs. Produce a grape, or corn tortilla-like odor.

45
Q
A

ASCP M Mometrix (18 decks)

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