BIOSAFETY LEVEL 3 PATHOGENS AND SELECT AGENTS (BIOTERRORISM)

Question 1

what are BSC3 pathogens?

Answer 1

pathogens that have the potential to cause serious or fatal disease through aerosals

Question 2

what are common BSC3 organisms?

Answer 2

-tuberculosis
-mold stages of systemic fungi

Question 3

what clinical specimens are used to test BSC3 organisms?

Answer 3

• Fluid and tissue from cutaneous lesions
• Stool
• Blood
• Sputum

Question 4

what are the biochemical reactions of Bacillus anthracis?

Answer 4

Catalase +
Oxidase −
Urease −
TSI K/K
H2S −
Motility −
Indole +
Nitrate reduction +

Question 5

how does B. anthracis appear on gram stain?

Answer 5

GP spore-forming bacilli in a long chain
“bamboo shoots”

Question 6

what are the growth requirements for B. anthracis?

Answer 6

aerobic, and facultative anaerobe, grow best at 35–37 °C

Question 7

what is the colony morphology of B. anthracis?

Answer 7

nonhemolytic, dry, ground glass surface, with irregular edges, and
comma protrusions called “Medusa head” on sheep blood agar

Question 8

what is the rapid test for B. anthracis?