Quiz 9,5 - 5th of june Flashcards
Amylase is.. that looks like…
It is a Ca2+ -dependent metalloenzyme involved in digestion, looks as shown in the picture
Where is amylase produced/secreted from?
secreted by salivary glands and the pancreas
In what pH does amylase achieve optimum enzymatic activity?
optimum enzymatic activity in slightly alkaline pH (7 - 8)
Dog breed differences in pancreatic amylase (AMY2)
In most dog breeds, an average of diploid (2n) AMY2=11 is observed,
while in dog breeds from Australia and the Arctic, diploid (2n) AMY2=3 is observed.
When starch is liquefied as a result of α-amylase action, what is released?
oligosaccharides are released
Determination of amylase in blood or urine helps diagnose…
acute pancreatitis, this must also be supplemented with the determination of pancreatic lipase
How is the activity of amylase determined?
The activity of amylase is determined based on the decrease in the intensity of the starch-iodine color reaction (iodine test)
What two forms does starch exist in?
- Linear (Amylose)
- Branched (Amylopectin)
The extracellular enzymes α-Amylase & Oligo-1,6-glucosidase are able to hydrolyze starch by…?
Breaking the glycosidic linkages b/w the sugar subunits
What is the end result of α-Amylase & Oligo-1,6-glucosidase hydrolyzing starch?
the complete hydrolysis of the polysaccharide to it’s individual α-glucose subunits
What reagent is used to detect the presence or absence of starch in the vicinity around the bacterial growth?
Iodine
what happens when the pancreas is diseased or inflamed?
amylase releases into the blood.
