Quiz 4 - 10th of april

Question 1

Saturated fatty acid

Answer 1

Question 2

Unsaturated fatty acid

Answer 2

Question 3

palmitic acid

Answer 3

C16

Question 4

stearic acid

Answer 4

C18

Question 5

oleic acid

Answer 5

C18, double bond on 9

Question 6

arachidonic acid

Answer 6

C20, double bond on C6, C9, C12, C15

Question 7

w-3 and w-6 fatty acids

Answer 7

Double bonds on either C3 or C6

Question 8

cardiolipin

Answer 8

Top:
Top C18 with double bonds at C9-C10 and C12-C13

Bottom C18 with double bonds at C9-C10 and C12-C13

C3 connected between phosphate groups, OH on side of C2

Bottom is a mirror image of top

Question 9

phosphatidylserine

Answer 9

top C18 with double bond at C9 and C10

Bottom C18 with double bond at C9 and C10

Question 10

phosphatidylcholine

Answer 10

Top C16.

Bottom C18 with double bond at C9 and C10

Question 11

phosphatidylinositol

Answer 11

top C16.

bottom C16

Question 12

cholesterol

Answer 12

3 hexagons, 1 pentagon, C5 and 2 branches at the end

Question 13

What is the lowry method?

Answer 13

The Lowry method is a combination of the biuret reaction and the reaction given by the Folin-Ciocalteu reagent (a complex of phosphomolybdic and phosphotungstic acids) with tyrosine, tryptophan and probably cysteine and histidine residues present in the protein. This results in blue-coloured products, whose absorbance is read at 750 nm. This allows the determination of proteins at concentrations of 1 ug/ml and above. Due to the differences in amino acid composition, different proteins give different colors with the Folin-Ciocalteu reagent, so it is important to use the corresponding standard for the protein under investigation.