Qualitative Tests for Amino Acids Flashcards

1
Q

For aromatics

A

Xanthoproteic

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2
Q

For peptide bonds

A

Biuret

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3
Q

For free amino acids

A

Ninhydrin

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4
Q

For indole

A

Hopkins-Cole

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5
Q

For phenolic compounds

A

Millon’s

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6
Q

For tyrosine and histidine

A

Pauly’s

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7
Q

For thiol group

A

Nitroprusside

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8
Q

For guanidine group

A

Sakaguchi

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9
Q

For S-containing compounds

A

Fohl’s

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10
Q

Reagent for Xanthoproteic

A

Nitric acid

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11
Q

Reagent for Biuret

A

Cupric sulfate

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12
Q

Reagent for Ninhydrin

A

Ninhydrin

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13
Q

Reagent for Hopkins-Cole

A

Glyoxylic acid

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14
Q

Reagent for Millon’s

A

Mercury in nitric acid

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15
Q

Reagent for Pauly’s

A

Diazotized sulfanilic acid

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16
Q

Reagent for Nitroprusside

A

Sodium nitroprusside

17
Q

Reagent for Sakaguchi

A

alpha-naphthol

18
Q

Reagent for Fohl’s

A

Lead II acetate

19
Q

Result of Xanthoproteic

A

Yellow

20
Q

Result of Biuret

A

Violet

21
Q

Result of Ninhydrin

A

Violet

22
Q

Result of Hopkins-Cole

A

Violet ring

23
Q

Result of Millon’s

A

Rose/Salmon

24
Q

Result of Pauly’s

A

Red

25
Q

Result of Nitroprusside

A

Red

26
Q

Result of Sakaguchi

A

Reddish/Wine

27
Q

Result of Fohl’s

A

Brown or black ppt