Pyruvate Dehydrogenase Complex Flashcards
What is the function of the pyruvate dehydrogenase complex from E. coli
Why is decarboxylating pyruvate so difficult
What is the role of TPP in the PDH complex
What are the 3 steps for the conversion of pyruvate to acetyl CoA
Give an overview of what happens in the PDH complex
Draw the mechanism for what happens in the E1 enzyme of the PDH complex
Base attached to E1
What happens to the intermediate produced in E1
Draw the mechanism for what happens in the E2 complex
What does coenzyme A do in the PDH complex
Give an overview of what happens in E2
Give an overview of what happens in E3
What are the final 2 cofactors used in the PDH complex
Draw the full Mechanism of oxidation of dihydrolipoamide catalyzed by E3 of PDH complex
Summarise the reactions that occur in the PDH complex
Why is having a large enzyme complex important
What is substrate channeling
What is a metabolon
is a temporary structural-functional complex formed between sequential enzymes of a metabolic pathway, held together both by non-covalent interactions and by structural elements of the cell, such as integral membrane proteins and proteins of the cytoskeleton
Give overall mechanism for what happens in the PDH complex
