Pyruvate Dehydrogenase Complex

Question 1

What is the function of the pyruvate dehydrogenase complex from E. coli

Answer 1

Question 2

Why is decarboxylating pyruvate so difficult

Answer 2

Question 3

What is the role of TPP in the PDH complex

Answer 3

Question 4

What are the 3 steps for the conversion of pyruvate to acetyl CoA

Answer 4

Question 5

Give an overview of what happens in the PDH complex

Answer 5

Question 6

Draw the mechanism for what happens in the E1 enzyme of the PDH complex

Answer 6

Base attached to E1

Question 7

What happens to the intermediate produced in E1

Answer 7

Question 8

Draw the mechanism for what happens in the E2 complex

Answer 8

Question 9

What does coenzyme A do in the PDH complex

Answer 9

Question 10

Give an overview of what happens in E2

Answer 10

Question 11

Give an overview of what happens in E3

Answer 11

Question 12

What are the final 2 cofactors used in the PDH complex

Answer 12

Question 13

Draw the full Mechanism of oxidation of dihydrolipoamide catalyzed by E3 of PDH complex

Answer 13

Question 14

Summarise the reactions that occur in the PDH complex

Answer 14

Question 15

Why is having a large enzyme complex important

Answer 15

Question 16

What is substrate channeling

Answer 16

Question 17

What is a metabolon

Answer 17

is a temporary structural-functional complex formed between sequential enzymes of a metabolic pathway, held together both by non-covalent interactions and by structural elements of the cell, such as integral membrane proteins and proteins of the cytoskeleton

Question 18

Give overall mechanism for what happens in the PDH complex

Answer 18