Pure Culture Techniques

Question 1

usually exist in mixed populations in their natural habitat

Answer 1

microorganisms

Question 2

n order to study a certain type of microorganism, it must be isolated to

Answer 2

pure culture

Question 3

s a population of cells which have originated from a single cell and, thus, are identical and of the same kind and species.

Answer 3

pure culture

Question 4

To obtain and maintain the purity of the culture, it is necessary to

Answer 4

prevent contamination or entry of unwanted organisms

Question 5

Different methods can be employed for the isolation of microorganisms to pure culture such as

Answer 5

plating
enrichment
serial dilution
single cell isolation

Question 6

plating techniques

Answer 6

streak plating
spread plating
pour plating

Question 7

draw the streaking pattern

Answer 7

+1

Question 8

. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of

Answer 8

droplets of condensed moisture

Question 9

different methods of isolation onclude

Answer 9

plating
enrichment
serial dilution
single cell isolation

Question 10

A common method for the isolation of a pure culture from a mixture is by “___” plates.

Answer 10

streaking

Question 11

draw the inoculum streaking pattern

Answer 11

+1

Question 12

why is there a need for cultivating bacteria?

Answer 12

one of the major aims of microbiology is to establish the distinctiveness of a particular bacterial species from any other species

Question 13

bacteria require what factors from the environment

Answer 13

energy source
macroelements
trace elements
growth factosr

Question 14

energy source of bacteria

Answer 14

sunlight
chemical compounds
organic matter

Question 15

macroelements of bacteria

Answer 15

carbon
hydrogen
oxygen
nitrogen
phosphorus
sulfur
potassium
calcium
magnesium

Question 16

trace elements of bacteria

Answer 16

manganese
copper
iron

Question 17

growth factor of bacteria

Answer 17

amino acid
purine
pyrimidine
vitamin

Question 18

two categories of media

Answer 18

chemically defined
chemically complex

Question 19

forms of media

Answer 19

solid
liquid

Question 20

types of solid form agar

Answer 20

test tube butt
test tube slant
agar plate

Question 21

types of liquid form of agar

Answer 21

tube broth

Question 22

sterilization can be two ways:

Answer 22

oven
autoclave

Question 23

in order to adequately study and characterize an individual bacterial species, one needs a ___ ___

Answer 23

pure culture

Question 24

bacterial suspension consisting of single species

Answer 24

pure culture

Question 25

techniques used to isolate culture

Answer 25

streak plating spread plating pour plating

Question 26

streak plate method includes the ff materials

Answer 26

inoculating needle mixed culture NA plate

Question 27

how many inoculum for spread plating

Answer 27

0.1 ml

Question 28

how many inoculum for pour plating

Answer 28

1 ml

Question 29

are fungi which exist predominantly or solely in a unicellular state

Answer 29

yeast

Question 30

where is yeast found

Answer 30

surfaces of plants and are abundant on the leaves, fruits and in the nectaries of flowers

Question 31

where is yeast found in human

Answer 31

skin mouth gastrointestinal tracts

Question 32

Yeasts can form

Answer 32

true mycelium pseudomycelium

Question 33

is a mycelium-like structure consisting of chains of cells formed by sequential budding (sprouting).

Answer 33

pseudomycelium