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BACTERIOLOGY LECTURE > PSEUDOMONAS, VIBRIO, HAEMOPHILUS, SPIROCHETES AND MISCELLANEOUS BACTERIA > Flashcards

PSEUDOMONAS, VIBRIO, HAEMOPHILUS, SPIROCHETES AND MISCELLANEOUS BACTERIA Flashcards

1
Q

An opportunistic pathogen that infects burns, wounds, surgical incisions, and sites of catheterization.

A

Pseudomonas aeruginosa

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2
Q

It is the most common cause of infections of burns injuries and outer ear (otitis externa)

A

Pseudomonas aeruginosa

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3
Q

What is the shape of Pseudomonas aeruginosa

A

Slender, rod-shaped (bacillus)

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4
Q

What is the arrangement of cells of Pseudomonas aeruginosa

A

Singles or in pairs

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5
Q

Is Pseudomonas aeruginosa non-spore-forming or spore-forming

A

Non-spore-forming

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6
Q

The gram-staining reaction of Pseudomonas aeruginosa

A

Gram-negative

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7
Q

What is the flagella of Pseudomonas aeruginosa

A

Amphitrichous

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8
Q

The special requirements of Pseudomonas aeruginosa

A

No complex special requirements

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9
Q

What is the wide range temperature of Pseudomonas aeruginosa

A

4-40 degC

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10
Q

The optimum temperature of most strains of Pseudomonas aeruginosa

A

37 degC

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11
Q

Oxygen requirement of Pseudomonas aeruginosa

A

Obligate aerobe

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12
Q

A selective medium for Pseudomonas spp.

A

Cetrimide Agar Medium

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13
Q

Cetrimide Agar medium inhibits the growth of …

A

Gram-positive bacteria

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14
Q

The shape of Pseudomonas on Nutrient Agar Medium

A

Irregular

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15
Q

The shape of Pseudomonas on Cetrimide Agar Medium

A

Circular

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16
Q

The shape of Pseudomonas on MacConkey Agar Medium

A

Circular

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17
Q

Color of Pseudomonas of Cetrimide Agar Medium

A

Greenish blue

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18
Q

Type of hemolysis of Pseudomonas on Blood Agar Plate

A

B-hemolysis

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19
Q

What culture is used for Pseudomonas that produces dense turbidity in broth medium with a surface pellicle

A

Trypticase soy broth/ Nutrient broth

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20
Q

What culture is used for Pseudomonas that produces a characteristics of sweetish odor or a distinctive musty or earthy smell

A

Nutrient Agar

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21
Q

What culture is used for Pseudomonas that produces colorless colonies, NLF

A

MacConkey Agar

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22
Q

On Cetrimide Agar Pseudomonas produces irregular colony growth due to …

A

Swarming of the bacterium

23
Q

Enumerate the 4 pigments produced by Pseudomonas

A

Pyocyanin
Fluorescein
Pyorubicin
Pyomelanin

24
Q

The color of the pigment of Pyocyanin

A

Bluish-green

25
The color of the pigment of Fluorescein
Greenish-Yellow
26
The color of the pigment of Pyorubicin
Reddish-brown
27
The color of the pigment of Pyomelanin
Brown to black
28
What are the characteristics of Vibrio
Gram-negative rigid curved rods comma-shaped
29
This organism is present in marine environments and surface water with cosmopolitan distribution
Vibrio
30
The most medically important member of genus Vibrio
Vibrio cholerae
31
Vibrio cholerae is the causative agent of ...
Diarrheal disease cholera
32
It is characterized by sudden effortless vomiting, profuse rice-water stool, followed by rapid dehydration
Diarrheal disease cholera
33
Two types of organisms under Vibrio cholerae
Vibrio cholerae (Non-O1) and Vibrio cholerae (O1)
34
Strains under Biotype
Classical El Tor
35
Strains under Toxin
Toxigenic Non-toxigenic
36
Oxygen requirement of Vibrio
Facultative anaerobe
37
The growth of Vibrio is stimulated by
Sodium chloride
38
pH range of Vibrio
6.4 - 9.6
39
Optimum pH range of Vibrio
8.2
40
Temperature range of Vibrio
16 - 40 degC
41
Optimum temperature of Vibrio
37 degC
42
Vibrio resembles the ...
Darting motility Swarm of gnats
43
The culture medium of Vibrio cholerae
Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)
44
Vibrio cholerae produces what color on (TCBS)
Yellow color
45
Vibrio cholerae produces yellow color on (TCBS) due to
Sucrose fermentation
46
What organism produces greenish color on TCBS
Vibrio parahaemolyticus
47
Rapid growth occurs in about 6 hours with the formation of thick surface pellicle
Alkaline peptone water (pH8.5)
48
Biochemical reactions of Vibrio cholerae
Ferments carbohydrates, producing acid with no gas Indole + Catalase & Oxidase + Methyl red & Urease -
49
This test can be used to identify Vibrio cholera colonies
String test
50
Reagent of String test
0.5% Sodium desoxycholate
51
Result of Vibrio cholerae on KIA
K/A
52
Result of Vibrio cholerae on TSI
A/A
53
This organism is considered as flesh-eating bacteria
Vibrio vulnificus
54
This organism can be acquired through shark bite
Vibrio harveyi

BACTERIOLOGY LECTURE (6 decks)

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