Pseudomonas aeruginosa Flashcards

1
Q

Environment

A
  • Soil
  • Water
  • Within Human/Animal impacted areas
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2
Q

Morphology

A
  • Gram +
  • Rod Shaped
  • Flagellated
  • Pili
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3
Q

Metabolic Properties

A
  • Heterotroph (carbon compounds)
  • Facultative anaerobe (prefers 02)
  • Nitrate ( terminal electron acceptor)
  • May ferment arginine
  • Psychrotolerant
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4
Q

Genomes

A

Core
Pan

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5
Q

Core Genome

A

Contains genes essential to the species’ survival.

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6
Q

Pan Genome

A

The total of all genes any strains may have that contribute to survival and growth.

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7
Q

Opportunistic pathogen

A

Invades people with weak immune systems
- immunocompromised
- chronically ill
- cystic fibrosis
- burns patients

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8
Q

WHO priority pathogen

A

Carbapenemase Resistant Strains

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9
Q

Characteristics of Virulence

A
  1. Surface Structures
  2. Lipopolysaccharide
  3. Secretion of toxins/virulence factors
    4.Quorum Sensing
  4. Biofilm formation
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10
Q

Surface Structures

A

Resist phagocytosis + Immune responses to induce inflammation

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11
Q

Lipopolysaccharide

A

contains O-antigen - increases the negative charge of cells, provides structural integrity and protects from different chemicals.
(Lipid A + A/B antigens)

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12
Q

Secretion of toxins

A

A prolific producer of exoproducts, secretion pathways export from the cytoplasm to the external environment, 5 different secretion systems.

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13
Q

Quorum Sensing

A

Communication between bacteria, monitoring cell density, secretion and biofilm formation may only occur when the population reaches a certain size.

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14
Q

Biofilm Formation

A

Aggregate forms where exopolysaccharide surrounds the cell.

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15
Q

Role of flagella

A

May help with binding to mucin found in cystic sputum

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16
Q

Role of pili

A

Help bacteria bind to epithelial cells

17
Q

Molecules Secreted

A
  1. Proteases
  2. Exotoxins
  3. Hydrogen Cyanide
18
Q

Biofilms - Antimicrobial Resistance

A

Aggregate forms where exopolysaccharide surrounds the cells and resists stressors such as bacteriophage host immune components and antibiotics.

19
Q

Antimicrobial Resistance Components

A
  1. Cell envelope.
  2. Secreted polysaccharide.
  3. Efflux Pumps.
20
Q

Genes associated with resistance may be acquired by?

A
  1. Horizontal Gene Transfer
  2. Mutations in Genes already in the chromosome.