Pseudomonas Flashcards

1
Q

caractere generale

A

bacili gram -
nefermentativi
mobili => 1 cil polar, subtri, drepti sau usor rotunjiti
energia este furnizata prin metab respirator si oxidativ al carbohidratilor
strict aerobi
nesporulati
bacili dispusi cap la cap => aspect de o singura celula
habitat = sol apa si mediul spitalicesc(medii umede)
metabolsim flexibil
oportunisti primari => pacienti imunodepresati
capabili de a folosi multe resurse de C si N

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2
Q

virulenta si patogenitate

A

structuri somatice :
LPZ - lipidul A - enterotoxina
pili
capsula
structuri extracelulare : enzime si toxine
enzime : neuraminidaza => indep resturile de a sialic de la niv recep piliari
proteaze alcaline, elastaze si hemolizine(lecitinaza, fosfolipaza C) => degradeaza matricea extracelulara si au efect citotoxic
toxine
exotoxina A -> inhiba sinteza proteica prin blocarea lantului peptidic
exotoxinele S si T => invazie tisulara
piocianina => formeaza specii de O toxice
pioverdina => siderofor care leaga Fe necesar metabolismului
poate forma biofilm

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3
Q

ce infectii produse

A

ale tractului resp inferior
ale pielii (plagi chirurghicale, arsuri )
oculare, otite
resp cronice - fibroza chistica
endocardite si meningite la imunosupresati
septicemie

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4
Q

caract de cultura

A

piocianina - pigment albastru verzui, specific P aeruginosa
pioverdina - pigemnt galbui verzui, produs de P aeruginosa
eritrina/ piorubina - pig rosu
pigementii sunt mai bine sintetizati in anaerobioza
miros aromat

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5
Q

in ce produse patologice

A
sange 
lichide din seroase
LCR
urina
bila 
sputa
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6
Q

izolare

A

bulion/apa peptonata 1%
geloza inclinata 2%
geloza sange
Mac Conkey

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7
Q

identificare pe geloza simpla

A
colonii rotunde
convexe 
fluorescente
reflexe metalice
miros aromatic
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8
Q

identificare bulion/apa peptonata

A

tulburare cu forma de penicula la suprafata mediului
se fragmenteaza si cade la fundul tubului
mediul se pigementeaza in albastru-verzui
miros de flori de tei

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9
Q

identificare Istrati Meitert

A

colonii mici albastre

aderente de mediu

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10
Q

identificare geloza sange

A

hemoliza beta

colonii micii si lucioase

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11
Q

caract biochimice

A
catalaza +
oxidaza +
fermenteaza glucoza
ureaza -
H2S -
indol-
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12
Q

tratament

A

imipenem tircacilina

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13
Q

imunoprofilaxie

A

vaccin piocianic polivalent

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