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Microbiology > Protues-Providencia-Morganella > Flashcards

Protues-Providencia-Morganella Flashcards

0
Q

Actively motile at 37C, pleomorphic,NLF, urease(+), Phenlyalanine Deaminase(+); Rapidly hydrolyzes urease at 2-4hours. Surface growth that gives off a BURNT GUNPOWDER ODOR; Swarming motile on BAP

A

PROTEUS

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1
Q

Different strains of proteus, when inoculated in culture medium swarms toward each other, but do not mingle thus leaving a demarcation line between them.

A

DIENESPHENOMENON

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2
Q

Pathogens of proteus

A

Wound and Urinary Tract Infection, nosocomial infection, bacteremia, pneumonia

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3
Q

Differenting point of P.mirabilis and P.vulgaris

A

INDOLE

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4
Q

Most important member of proteus genus. Susceptible to ampicillin, chloramphenicol, cephalosporin. OX-K(kingsburry); may cause pneumonia and septicemia. Indole (-). Tend to swarm on moist agar producing bluish gray confluent.

A

PROTEUS MIRABILIS

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5
Q

Targets the immunosuppressed individuals; OX-02 and OX-19; INDOLE (+); may cause nosocomial infections and uti. Resistant to Ampicillin, chloramphenicol, cephalosporin. Has the same antigenic structure as rickettsiae such that O antigen

A

PROTEUS VULGARIS

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6
Q

To detect rickettsial infections.

A

WEIL-FELIX TEST

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7
Q

Most important species of morganella. Causes UTI and wound infections as well as diarrhea. Lactose, citrate, h2s and LDC(-); urease and Deaminase (+). Formerly known as P.morganii

A

MORGANELLA MORGANII

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8
Q

Normal intestinal flora; difficult to treat because most of them are R to antibiotics. Associated with nosocomial infection, gram -, NLF, pleomorphic, motile at 25 , non motile at 31c. Urease - and NLF

A

PROVIDENCIA

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9
Q

Associated with some cases of diarrhea in children. Infection are rare. Do not swarm on BAP.

A

PROVIDENCIA ALCALIFACIENS

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10
Q

Other Providencia species

A

PROVIDENCIA STUARTII, PROVIDENCIA RETTGERI, PROVIDENCIA RUTSIGANII.

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11
Q

NLF, microaerophilic, short coccobacilli, exhibits bipolar granules, ferments sucrose.

A

YERSINIA

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12
Q

Yersinia on Desoxycholate agar

A

REDDISH PINK COLONIES; DOES NOT FERMENTS LACTOSE; AEROGENIC

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13
Q

Yersinia on old culture

A

ORGANISM FORMS SURFACE GROWTH PELLICLE WITH STALACTITE STREAMERS

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14
Q

Virulence factors of Yersinia.

A

ENDOTOXINS, COAGULASE, FIBRINOLYSIN

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15
Q

Two most important species of Yersinia, that causes enterocilitis.

A

Y.enterocolitica and Y.pestis

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16
Q

Causative agent of Y.pseudotuberculosis or Y.enterocolitis; can cause a severe intestinal inflammation

A

Yersiniosis

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17
Q

Opportunistic pathogens of Yersinia.

A

Y.intermedia, Y.freduikaenii, Y.kristensenii

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18
Q

Most common Yersinia isolated from human. Causes enterocolitis. Most often encountered Yersinia in the lab. Invasive pathogen which can penetrate gut lining and enter the lymphatic system and blood. Infections are usually through an ingestion of contaminated food, which can cause a severe intestinal inflammation. Release of it’s enterotoxin cause a severe pain similar to that found in patients with appendicitis. Easily found because it has the ability to grown in a cold temp and motile at room temp.

A

Y.enterocolitica

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19
Q

Also known as plaque bacillus.

A

Y.pestis

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20
Q

Infection of wild rodents transmitted occasionally by bite of fleas

A

Plague

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21
Q

From oriental rat flea bite. Symptoms niya is swelling of lymph nodes. Nagcacause ng cell death and black purpuric lesions or blak death.

A

Xenopsylla cheopsis

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22
Q

3 human plague in Y.pestis. Lymph nodes. Buboes

A

Bubonic plague

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23
Q

3 human plague in Y.pestis. Airborne transmission

A

Pnuemonic plague

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24
Q

3 human plague in Y.pestis. Black Death due to Schwartzmann phenomenon.

A

Septicemic plague

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25
Q

Mesetric lymphadenitis, septicemia. LOA –, Urease +, ODC and sucrose -.

A

Y.psuedotuberculosis

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26
Q

Stained smears of Y.pseudotuberculosis.

A

Wayson and Giema stain.

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27
Q

Culture of MAC on Y.pseudotuberculosis.

A

Colorless to peach

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28
Q

Culture of emb in y.pseudotuberculosis.

A

Colorless to purple

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29
Q

Culture of HEA on y.pseudotuberculosis.

A

Salmon

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30
Q

Culture of XLD in Y.pseudotuberculosis

A

Yellow

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31
Q

Culture of SSA on Y.pseudotuberculosis.

A

Purple

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32
Q

Transport medium for Y.pseudotuberculosis. For transporting and maintaining tissue infected with Y.pestis, also useful for transporting stool for isolation of shigella, salmonella and Yersinia.

A

Cary-Blaire medium

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33
Q

Found in brackish or salt water. Short, facultative anaerobes, ferments glucose, NLF, reduce nitrates to nitrites, Oxidase + except V.metschnikovii, INDOLE +, catalase +, LOA ++-. Hemophilic organism except V.cholera and V.mimicus, natural habitat is water, usually associated with seafood or dairy. Motile, comma/curved bacillus. Not an enteric gastrointestinal pathogen but has the ability to cause gastroenteritis.

A

Family Vibrionaceae

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34
Q

Members of Family Vibrionaceae.

A

Vibrio, Aermonas, Plesiomonas

35
Q

Vibrio comma. Curve/comma shaped rods, gram - rods, facultative anaerobes, non halophilic ferments glucos

A

Vibrio cholerae

36
Q

V.cholerae under contrast or dark field microscopy

A

Darting motility or shooting star motility

37
Q

Potent enterotoxin on V.cholerae

A

Cholera toxin, zit toxin, ace toxin.

38
Q

Cholera toxin in V.cholerae

A

Cholerogen 01 and 0139

39
Q

String test of V.cholerae

A

Reagent: 0.5% sodium desoxycholate +; sucrose fermenters (yellow TCBS)

40
Q

Transport medium of V.cholerae

A

Amies medium

41
Q

Enrichment medium of V.cholerae

A

Alkaline Peptone Water

42
Q

Culture of V.cholerae on BAP

A

Smooth, medium to large colonies with greenish blue

43
Q

V.cholerae on TCBS

A

pH 8.4. Yellow colonies

44
Q

Disease characterized by loss of large amounts of fluids

A

Asiatic Cholera

45
Q

Toxin in V. Cholerae that is a rice watery stool

A

Endotoxins

46
Q

Toxin in V.cholerae that is found in chromosome

A

Enterotoxin

47
Q

Causative agent of V. Cholera

A

V. Cholera eltor

48
Q

Serological type: V. Cholerae 01 serotypes: found in the Phil.

A

Original J or Inaba type

49
Q

Serological type: V. Cholerae 01 serotypes: found in Japan

A

Middle intermediate or Hikojjma type

50
Q

Serological type: V. Cholerae 01 serotypes: in India

A

Variant F or Ogawa type

51
Q

Lysis of v. Cholerae when inoculated into an immuned guinea pigs

A

Bacteriolysis

52
Q

Phenomenon of Bacteriolysis

A

Pfeiffer’s Phenomenon

53
Q

Classical is - and Eltor +

A

Bio types: RBC hemolysis, VP, aggtn. With chicken RBC

54
Q

Classical s and Eltor is r

A

Bio type polymixin B

55
Q

Halophilic organisms causing gastroenteritis and food poisoning associated with consumption of a contaminated seafoods.

A

Vibrio Parahaemolyticus

56
Q

Factors causing hemolysin

A

Heat stable and labile direct hemolysin, phospholipase A and Lysophospholipase

57
Q

Phenomenon of V. Parahaemolyticus

A

Kaganawa Phenomenon

58
Q

Ear and wound infection. Associated with marine environment, halophilic, sucrose +, yellow on TCBS.

A

V. Aglinolyticus

59
Q

Septecimia, wound infection involving marine environment. Known to cause necrotizing fasciitis, extremely virulent, green on TCBS. Associated with eating raw oyster.

A

V. Vulnificus

60
Q

Wound infection

A

V. Damsela

61
Q

Food poisoning

A

V. Fluvialis

62
Q

Food poisoning, non marine vibrio, green on TCBS, non halophilic, sucrose -

A

V. Mimicus

63
Q

Facultative anaerobes, oxidase and catalase +, motile but some species are not motile. Bull’s eye colonies on CIN, beta-hemolytic on BAP, can grown on Mac, EMB, SSA, CIN. Isolated from tap water, soil, river, marine environment and various foods. Halophilic. Pathogens of cold blooded animals such as frogs and snakes.

A

Aeromonas

64
Q

Causes gastroenteritis and cellulitis

A

A. Sobra and A. Hyrdophilia

65
Q

Water loving organism, associated with gastrointestinal disease

A

Aeromonas hydrophilia

66
Q

Characteristics that separated it from other member of Enterobacteriaceae. Oxidase +

A

Plesiomonas

67
Q

Biochemically similar to shigella. Motile, oxidase +, do not produce gas. Can grow on media that used for enteric bacteria, found in fresh water especially in warmer climates. Glucose fermenters and genetically related to Proteus. Exposure to cold blooded animals such as reptiles and may cause gastritis.

A

Plesiomonas Shigelloides

68
Q

Species used for P.shigelloides

A

Stool and rectal swab

69
Q

Separates Vibrio spp. From Aeromonas sp and P.shigelloides

A

String test: 0.5% sodium desoxycholate

70
Q

Differentiated V. Cholerae 01 and non 01 from other species

A

Vibrio static test- 0129

71
Q

Small spirals, s shaped, sea gull winged forms, sensitive to h2o2 and superoxide, oxidase +, catalase +, urease -, no3 +, gram -, monotrichous flagella, non-CHO fermenters, unable to grow on 3.5% NaCl. Most common cause of gastroenteritis in the US. Also cause septicarthitis. Most recognized antecedent cause of gullain Barre syndrome.

A

Campylobacter Jejuni

72
Q

Susceptible in nalidix acid, resistant in cephalosporin, able to hydrolyzes hippurate.

A

Camplyobacter Jejuni

73
Q

Optimal Incubator temp of c. Jejuni

A

42C

74
Q

Specimens of CampylobacterJejuni

A

Feces, rectal swab and blood

75
Q

Other species of Campylobacter

A

C. Jejuni and C. Coli

76
Q

Animal pathogen that causes animal abortion

A

C. Lari

77
Q

Serolgy of campylobacter Jejuni: for soluble heat stable agglutination

A

Penner method

78
Q

Heat labile with slide agglutination tech. C. Laridis, C. Coli, C. Jejuni

A

Lior Method

79
Q

Formerly vibrios fetus

A

Campylobacter Fetus

80
Q

Transport media for C.Fetus

A

Buffered saline glycerol and alkaline Peptone water.

81
Q

Formerly identified as C.pylori, spiral shaped resembling Campylobacter . NS. Causes type B gastritis, peptic ulcer, gastric carcinoma, bad breath and cancer. Rodlike bizarre, u shaped gr(-) bacteria, tuft or polar flagella with corkscrew motility. Strongly urease and catalase +.

A

Helicobacter pylori

82
Q

Specimens used for Helicobacter pylori

A

Urine and tissue biopsy material

83
Q

Differentiating point with campylobacter and Helicobacter

A

Motility with 4-6 flagella and optimum temp 35-37C.

84
Q

Excellent sensitivity and specificity lab diagnosis of Helicobacter pylori

A

Urea breath test

Microbiology (7 decks)

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