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Microbiology > Non Fermantative Gram Negative Bacilli > Flashcards

Non Fermantative Gram Negative Bacilli Flashcards

0
Q

Positive open tube; negative close tube. Non fermenter = Pseudomonas, Alcaligenes

A

Oxidizer or oxidative

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1
Q

Positive both open and close tubes. Fermenters = coliform organisms. E.coli

A

Fermentative (yellow color)

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2
Q

Can only use CHO only in the presence of air

A

Acinetobacter, stenotrophomonas

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3
Q

Both negative in open and close tubes

A

Non oxider

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4
Q

Unable to utilize CHO in the absence or presence of air

A

Non-saccharolytic

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5
Q

Sealed remains green, if unsealed becomes yellow

A

Oxidative fermentation

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6
Q

Slight yellowish on top of both tubes

A

Oxidative or slow fermentation

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7
Q

Widely R to most antibiotics; obligate aerobe, straight gram -, NS, catalase and oxidase +, metabolism is respiratory

A

Pseudomonas

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8
Q

Growth at 35C

A

+ Pseudomonas aeruginosa

- Pseudomonas flavescens

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9
Q

Cetrimide test at 35C for 7 days

A

+ Pseudomonas aeruginosa

- Escherichia Coli

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10
Q

Litmus milk test at 35c for 7 days

A

Acid - pink (E. Faecium) - coagulate casein

Alkaline - blue dot ( A. Faecaelis)

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11
Q

RNA morphology group of pseudomonas: RNA group 1

A

The fluorescens group: P. Aeruginosa, P. Fluorescens and P. Putida
The Stutzeri group: P. Stutzeri and P. Mendocina
The Alcaligenes group: P. Alcaligenes and P. Pseudoalcaligenes

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12
Q

RNA morphology group of pseudomonas: RNA group 2

A

The Pseudomallei group: P. Mallei, P. Pseudomallei and P. Cepacia

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13
Q

RNA morphology group of pseudomonas: RNA group 3

A

The Acidovorans group: P.acidovorans and P. Testosteroni

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14
Q

RNA morphology group of pseudomonas: RNA group 4

A

The Diminuta Group: P. Diminuta and P. Vesiculare

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15
Q

RNA morphology group of pseudomonas: RNA group 5

A

Xanthomonas ( Pseudomonas) maltophila

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16
Q

Used to detect organism type of action of CHO; commonly used by Pseudomonas; oxidize sugars in low levels, oxidase +, can grow in any lactose medium, motile. This test is designed to differentiate bacteria on the basis of fermentative or oxidative metabolism of CHO

A

Oxidative Fermentative Medium

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17
Q

Promotes anaerobic group due to true fermentation, incubate then add mineral oil.

A

Closed OF Medium

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18
Q

No mineral oil is added to allow aerobic growth and oxidation, changes are due to oxidative utilization of CHO present. Stab then incubate.

A

Open OF medium

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19
Q

pH indicator used:
Acid - yellow
Alkaline - blue

A

Bromythmol blue

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20
Q

MAC on Pseudomonas

A

NLF

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21
Q

Important member of Pseudomonas; Also known as Agent of blue pus and previously known as Bacillus pyocyaneus.

A

Pseudomonas aeruginosa

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22
Q

Morphology of Pseudomonas Aeruginosa

A

NS; NE; Motile (monotrichous) except B.mallei; aerobic with pili

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23
Q

Oppurtunistic pathogen of P.aeruginosa

A

Utilizes glucose oxidatively, oxidase + except S.maltophila, growth at 42C, Cetrimide +, NO3 +, Acetamide +

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24
Q

Causative agent of Pseudomonas Aeruginosa

A

Swimmer’s rash or ear; dermatitis “Jacuzzi or hot tub syndrome” and erythema gangrenosum, contact lens infection

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25
Q

Virulence factors of P.aeruginosa

A

Exotoxin A, endotoxins, slime polysaccharide, elastase and alkaline protease, alginate pilli.

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26
Q

Cultural characteristics of P.aeruginosa on BAP

A

Large flat colonies with ground glass appearance and a zone of hemolysis which is a beta hemolysis, bluish green pigment

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27
Q

2 distinct hemolysin of P. Aeruginosa

A

Heat labile phospholipase a

Heat stable glycolipid

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28
Q

Turquoise blue phenazine pigment which acts as antibiotic inhibiting growth of other org. Soluble in water, chloroform.

A

Pyocyanin

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29
Q

Water soluble; pyoverdin produces a yellowish green tinge

A

Flourescein

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30
Q

Red

A

Pyorubin

31
Q

Brown

A

Pyomelanin

32
Q

Bluish green.

A

Pyochelin

33
Q

Green

A

Pyoverdin

34
Q

Colonies of P.aeruginosa tends to give off what?

A

Musty odor

35
Q

Pathogenecity of P. Aeruginosa

A

Lipopolysaccharide, endotoxins, Exotoxin A and S. Phosphorylase Chemolysin, cytotoxin- leukocidin

36
Q

Gram stain of P. Aeruginosa

A

Straight and slender rods

37
Q

BAP, CAP, MAC and Seller’s medium in P.aerugionosa

A

Mucoid colonies; good growth at 42C.

38
Q

Fluorescein but not a pyocyanin; no growth at 42C; causes UTI, Bacteria and wound infection

A

Pseudomonas fluorescens

39
Q

Brown (buff colored) wrinkled colonies, NLF, 6.5% NaCl+

A

Pseudomonas Stutzeri

40
Q

K/K with H2S, NLF, oxidase+

A

Shewanella putrefaeciens

41
Q

Differentiating test that will seperate Burkholderia + and Stenotrophomonas -.

A

Oxidase test

42
Q

Generally non pathogenic, involved in human with heavy contaminated medical devices. Aerobic, oxidase +, catalase +, NS, motile except B.mallei

A

Burkholderia

43
Q

Species of Burkholderia

A

B.mallei, B.galliodis, B.pseudomallei and B.cepacia

44
Q

2 cause of cystic fibrosis. Causes foot rot to man, septicemia and pneumonia. Survives well in hospital environment, soil, plants, water and disinfectant. Earthy or dirty like odor. Oxidase and LDC+, motile and drug resistant. Pigment is yellow to yellow green. Previously know as P.kingii and P.multivorodans

A

Burkholderia cepacia

45
Q

Former known as P.mallei and common name is Whitmore’s bacillus. Cocoid-rod shaped, oxidase -, unable to grow at 42C, NM.

A

Burkholderia mallei

46
Q

Occasionally transmitted to man, horse’s disease

A

Glanders

47
Q

Formerly know as P.pseudomallei. Causes melliodis, septicemial glanders like disease, said to emit the earthy odors. Growth at 42C, M. Known as Vietnamese Time bomb

A

Burkholderia pseudomallei

48
Q

Media used in B.pseudomallei, which gives a dry, wrinkled, violet colonies

A

Ashdown culture medium

49
Q

Formerly known as Xanthophomonas mallei, not part of the human flora, maltose loving bacteria, Motile, utilizes glucose and maltose aerobically only. Grows on 42c, DNase - and OF +/-, colonized the burnt skin

A

Stenotrophomonas mallei

50
Q

BAP on Stenotrophomonas mallei

A

Lavender- green to light purple pigment with ammonia cal smell

51
Q

Motile, oxidase +, non fermantative , NLF, isolated in water and soil including hospital environment, acquired thru contaminated medical device and solution, feather edged colonies.

A

Alcaligenes faecalis

52
Q

BAP on Alcaligenes faecalis

A

Surrounded by a zone of green discoloration, alpha-hemolytic. Smell resembles apple or strawberries

53
Q

Member of the neisseriaceae family. Oxidase -, catalase+, NM, Mac-, grm-plump diplococci, + glucose medium, strict aerobe, - in nitrite test, cause UTI, wound and diarrhea. Acquired thru contaminated medical devices

A

Acinetobacter calcoaceticus

54
Q

Varieties of A.calcoaceticus: acid strains

A

A.calcoaceticus var anitratum (Herellea vagnicola)

55
Q

Varieties of A.calcoaceticus: non-acid strains

A

A.calcoaceticus var lwoffi (Mima polymorpha)

56
Q

Glucose oxidizing , non hemolytic (oxidizer)

A

A.baumaii

57
Q

Difference between A.lwoffi and N.gonorrhea

A

Is that A.lwoffi can grow on both EMB and Mac

58
Q

Coccobacilli, NLF, can grow on urethra

A

Oligella

59
Q

Oligella on BAP

A

Small opaque colonies

60
Q

NM, urea hydrolysis - and oxidase +

A

Oligella urethralis

61
Q

Motile, urea hyrdolysis and oxidase -

A

Oligella ureolytica

62
Q

Medium to long straight rods, oxidase, DNase, gelatin hyrdolysis and INDOLE +; NM

A

Chrysebacterium

63
Q

Before it was know as what? That may be transmitted from birth canal to neonate, may enscapulated, may cause UTI endocarditis and bacteremia

A

Chryseobacterium (Flavobacterium) meningosepticum

64
Q

Chryseobacterium (Flavobacterium) meningosepticum on BAP and CAP

A

Yellowish pigment

65
Q

New name of Chryseobacterium

A

Elizabethkingia meningosepticum

66
Q

Common name is Morax-Axenfeld; agent of blephanoconjunctivitis/angular conjunctivitis. Coccobacilli to medium rod, catalase +z

A

Moraxnella lacunata

67
Q

Moraxalle lacunata on MAC

A

No growth

68
Q

Normal flora of the Moraxella lacunata that inhibits the mucous membranes

A

Nose, throat, other parts of the URT and conjunctiva

69
Q

Oxidase +, catalas +, NM; grm-coccobacilli , asaccharolytic

A

Moraxella osloensis

70
Q

Differentiation test of Moraxella osloensis - and Neisseria gonorrhea +

A

CTA ( Cystine Tripticase Agar)

71
Q

Oxidase +

A

Alcaligenes, flavobacterium, Moraxella, kingella, eikenella

72
Q

Oxidase -

A

Acinetobacter

73
Q

Catalase +

A

Acinetobacter, Alcaligenes, Flavobacterium, Moraxella

74
Q

Catalase -

A

Kingella, eikenella

Microbiology (7 decks)

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