PROTOZOA (Intestinal Amoeba) Flashcards
Amoebic dysentery
o Intraintestinal
o Extraintestinal
(liver -> hepatic amoebiasis)
Extraintestinal
Trophozoite
o Cytoplasm
o Nucleus
clear, transparent, and refractile
Outer ectoplasm
finely granular, having a ground glasslike appearance
Inner endoplasm
contains ingested RBC
Endoplasm
spherical in size and contains central karyosome, surrounded by clear halo and anchored to the nuclear membrane by fine radiating fibrils cartwheel appearance
Nucleus
Cyst
o Chromatoidal
o Quadrinucleated
cigar shape
Chromatoidal bodies
Entamoeba histolytica trophozoite Distinguishing features
Uninucleated
Clear cytoplasm (fine granules)
Bull’s eye karyosome
Finger-like pseudopodia
Rapid unidirectional progressive motility
With ingested RBC
Differs in DNA and RNA content
Entamoeba dispar
Amoeba of pigs, Cyst is uninucleated
Entamoeba polecki
Small-reace of amoeba
Entamoeba hartmanni
Closest to E. histolytica in terms of size
E. hartmanni
Closest to E. histolytica in terms of overall appearance including genetic materials
E. dispar
Size (um) 12-60
E. histolytica (TROPHOZOITE)
Size (um) 20-50
E. coli (TROPHOZOITE)
Motility of E. histolytica trophozoite
Unidirectional / Progressive
Motility of E. coli trophozoite
Sluggish non directional
Pseudopodia of E. histolytica trophozoite
Long and finger like
Pseudopodia of E. coli trophozoite
Blunt and rounded
Cytoplasm of E. histolytica trophozoite
Ground glass appearance
Cytoplasm of E. coli trophozoite
Trashy
Inclusion of E. histolytica trophozoite
RBC
Inclusion of E. coli trophozoite
Bacteria and debris
Nucleus of E. histolytica trophozoite
Central but indistinct
Nucleus of E. coli trophozoite
Eccentric
Karyosome of E. histolytica trophozoite
Bull’s eye karyosome
Size (um) 10-15
E. histolytica cyst
Size (um) 10-30
E. coli cyst
Number of nucleus of E. histolytica cyst
4
Number of nucleus of E. coli cyst
8
Spherical shaped
E. histolytica cyst
Spherical or oval shaped
E. coli cyst
Chromatoidal bodies which are Cigar/Rod shape
E. histolytica cyst
Chromatoidal bodies which are Broom stick shape
E. coli cyst
Size (um) 6-15
E. nana trophozoite
Size (um) 6-20
I. butschillii trophozoite
Size (um) 5-25
E. gingivalis trophozoite
Pseudopodia (Bluntly rounded)
E. nana trophozoite
I. butschillii trophozoite
Pseudopodia (Multiple Bluntly rounded)
E. gingivalis trophozoite
Nuclear karyosome (Large, irregular blot-likeappearance)
E. nana trophozoite
Nuclear karyosome (Achromatic appearance)
I. butschillii trophozoite
Nuclear karyosome (Central)
E. gingivalis trophozoite
Movement (Sluggish Non-directional)
E. nana trophozoite
I. butschillii trophozoite
E. gingivalis trophozoite
Size (um) 5-14
E. nana cyst
Size (um) 5-18
I. butschillii cyst
Oval shaped
E. nana cyst
Irregular shaped
I. butschillii cyst
Number of nuclei (E. nana cyst)
4
Number of nuclei (I. butschillii cyst)
1
Chromotoidal body which are Small and Spherical
E. nana cyst