protiens Flashcards
proteins are made of
amino acids
amino acid parts
amino group
carboxyl
single hydrogen
r group
amino acids are linked through
peptide bonds
n terminal
first amino acid in sequence
c terminal
last amino acid in sequence
protein shapes are due to
ionic bonds
hydrophobic bonds
hydrogen bonds
disulfide (cystine only)
primary protein structure
amino acid sequencese
secondary struc
interactions of gorup in peptide backbone
tertiary structure
folded shape of single polypeptide chain
quaternary structure
interactions bn multiple polypeptide subunits
secondary structure motifs
alpha helix
beta sheet
alpha helices
twisty i guess
beta sheets
antiparrallel
parallel
function determines
folding pattern
enzyme folding creates
activation sites
enzyme role
lower actvation energy for a reaction by binding to active site
domain
independent folding region
more than 2nd, less than tertiary
like packets of structure
how do proteins/ polypeptides interact
with h bonds, ionic bonds, and hydrophobic
types of protein/ polypeptide interactions
surface surface
helix helix
surface string
x ray crystallography
need protein crystal for the process
needs diffraction patter
protein nmr
gets solution based structures
creates resonance peaks bn near neighbor atoms
cryo EM
generate micorgrapahs of a protein
structure get resolved by computer refinements
Alpha fold
ai protein structure generator
its accurate asf
denaturation
protein structure melts and funciton is lost
denaturation can be caused by
pH, temp, salt, hydration
nucelotides
make up of nucleic acids such as DNA and RNA
nucleotide fucntion
storgage, transmission, used of genetic info
nucleotide units
sugar, phosphate, base
nucelotides are linked with
phosphodiester bonds
polynucleotide strands are (orientation)
antiparallel and complementary
what forces hold ds DNA together
h bonds (base pairings)
hydrophobic forces (base stacking)
RNA
less stable than DNA
usaly one stranded
mRNA
provides codons for amino acid to be added to polypeptide
tRNA
carries an amino acid that corresponds to the ribosome
rRNA
part of the structure of the ribosome