Proteins

Question 1

Enzyme assays

Answer 1

Methods used to measure the rate/velocity of reaction of an enzyme

Question 2

Initial velocity

Answer 2

Vo measures the earliest part of the enzymatic reaction

Question 3

Active site

Answer 3

A specialised microenvironment where specificity and catalysis occur

Question 4

Chymotrypsib active site residues

Answer 4

Asp102-His57-Ser195

Question 5

Protease

Answer 5

Enzyme that cuts proteins into smaller fragments

Question 6

Hydrolysis

Answer 6

To break a chemical bond using water

Question 7

Inhibitor

Answer 7

A substance that interferes with an enzyme to slow it down e.g medicines

Question 8

Competitive inhibitor

Answer 8

Similar in structure to the substrate and competes for active site, resistant to action of enzyme , can be overcome of at high [S]

Question 9

Methotrexate

Answer 9

A competitive inhibitor of the enzyme DHFR

Question 10

Covalent catalysis

Answer 10

Involves a covalent bond between the enzyme and an intermediate in the reaction

Question 11

HIV aspartyl protease

Answer 11

Dimer of identical subunits required for virus production. Target for inhibitors used in anti-HIV therapy

Question 12

Secondary structure of proteins

Answer 12

The regularities by hydrogen bonding between carbonyl and amuse groups of the backbone to give alpha helices + beta sheets