Protein trafficking between membranes. Flashcards
What is the coat used for?
Budding and scission of vesicles.
What happens to the coat before tethering occurs?
It dissociates from the vesicle.
What member of the endomembrane is responsible for endocytosis?
Lysosomes
What member of the endomembrane is responsible for Excocytosis?
Endoplasmic reticulum and Golgi aparatus
What is the sequence of compartments proteins go through to leave a cell
Golgi
Vesicle
Membrane
What are the two processes for Exocytosis
Constitutive and regulated
What are common neurofiliments?
NF-L, NF-M, NF-H
Where are neurofiliment proteins found?
MTOC in neuronal cells.
What do Neurofilaments do?
Stabilize the organization of the endoplasmic reticulum and golgi complex
What is the regulator mechanism for endocytosis
Enzymes are released only when signalled by appropriate stimuli
Where do vesicles move in retrograde transport
From golgi to ER
How are vesicles exiting the endoplasmic reticulum formed?
COP II
Sorting proteins in cytoplasmic tail.
Soluble proteins eneter bulkflow or bind to signal proteins.
What is the targeting signal used to retain resident Golgi protein
Signal anchor and flank sequence.
What are SNARES used for
Docking
Where do COPI vesicles move proteins in forward transport?
From CGN to TGN (Cis golgi to trans golgi)
Where do COPI vesicles move proteins in reverse transport?
From VTC or Golgi to ER.
What is needed for COPI to do reverse transport?
KDEL sequence.
What are the ordered Steps prior to fusion of a vesicle
Budding Scission Uncoating Tethering Docking Fusion
What type of vesicle transport from Golgi to ER in retrograde transport
COPI
What would a cell lacking functional T-SNAREs look like
Docking wouldn’t happen
What is the complex that unwinds v/t-SNARES
NSF
What is the role of SNARE proteins?
To allow for fusion by docking.
Where does ATP hydrolysis occur in the process of fusion to a target membrane?
In unraveling snares via NSF
What does Hsc70 do in endocytosis
Uncoats
