Ch 9 Nucleus

Question 1

Is traffic through nuclear pore unidirectional?

Answer 1

No, import and export

Question 2

experiment to determine if NLS is sufficient for protein transport into the nucleus

Answer 2

Inject protein into cytoplasm and monitor movement of protein

Question 3

Define RNA and role

Answer 3

Ribosomal nucleic acid: code DNA

Question 4

Property of nuclear pore

Answer 4

Small opening in nuclear membrane

Question 5

How was nuclear pore discovered

Answer 5

Electron microscapy

Question 6

How does Leptomycin function in human cells?

Answer 6

Blocks nuclear export of REV by binding Crm 1 and disrupting ability to bind to Nuclear Export Signals (NES)

Question 7

2 Roles and Functions of IkB

Answer 7

Promotes Export, inhibits NF-kB

Question 8

T/F Nucleus is completely filled with chromatin

Answer 8

False

Question 9

What do Nuclear Lamin Proteins make?

Answer 9

Multiprotein Complexes

Question 10

How does tRNA export from nucleus and how is Ran involved.

Answer 10

tRNA exports via Ran-GTP. Ran-GAP inhibits Ran- GTP.

Question 11

Nucleus advantage

Answer 11

Regulation and cell differentiation

Question 12

Define Nucleolus

Answer 12

Region inside nucleus where ribosomes are produced.

Question 13

What protein binds to Nuclear Localization Signals (NLS) as part of nuclear protein transport

Answer 13

Cytoplasmic receptors

Question 14

What is Importin cargo

Answer 14

NLS

Question 15

What is exportin cargo

Answer 15

NES

Question 16

Define Heterochromatin

Answer 16

Highly condensed part of genome. Does not get transcribed.

Question 17

Feature of transport across Nuclear Envelope

Answer 17

Proteins move through nuclear pore via active transport

Question 18

What ensures that cargo-protein complex does not exit NPC after it enters the nucleus

Answer 18

Ran-GTP

Question 19

How is mRNA processing linked to MRNA export from nucleus

Answer 19

Proteins that transcribe mRNA Package it for export.