Protein Synthesis (9) Flashcards
during transcrption where are the nucleotides found ?
freely in the nucleus
what bases does RNA have ?
A
U
C
G
How does the template strand enable am mRNA trand tp be transcribed?
by using the complementary base pair rule
what is the function of RNA polymerase in transcription ?
to break apart the DNA strands
what enzyme breaks down nucleotides ?
DNAnuclease
where is the pre-mRNA cap placed ?
at the 5’
what is the cap composed of ?
phosphorylated 7-methyl guanosine
how is the cap placed on the end of the mRNA ?
by guanyltransferase
What 2 functions of the pre-mRNA cap ?
Blocks degradation of mRNA by 5’ exonucleases
promotes translation
what is the process called where a poly-A-tail is added to pre-mRNA ?
Polyadenylation
where on the pre-mRNA is the poly-A-tail added ?
at 3’
before the poly-A-tail is added to a pre-mRNA strand what must be done ?
pre-mRNA is cleaved near a signal AAUAAA at the 3’
what enzyme cleaves the pre-mRNA near the signal AAUAAA?
An endonuclease
approximately how many adenosine residues are added to the cleage site ?
200
by what enzyme are the adenosine residues added to the cleage site ?
poly-A polymerase
name 4 functions of the pre-mRNA poly-A-tail
protects the mRNA from degradation by 3’ exonucleases
also aids in termination of transcription
ensures export from the nucleus
and is important in translation
what is difference between an exon andd an intron ?
exons code for proteins and introns do not
how is final mRNA produced from pre-mRNA ?
Introns are spliced out
what can alternate splicing produce ?
Alternative splicing of pre-mRNA sequence can produce different proteins from the same gene
why is alternate splicing benificial for organisms ?
Less genes needed to code for more proteins
name 5 sections that mature mRNA contains
5’ cap 5’ UTR (untranslated region) Coding Region (to be translated into a protein) 3’ UTR (untranslated region) Poly(A) tail
by what bonds are amino acids bonded together ?
covalent bonds
which two amino acids are considered small ?
glycine
alanine
which three amino acids are consiered nucleophillic ?
serine
threonine
cysteine
name 5 amino acids that are considered hyrodrophobic
valine leucine isoleucine methionine proline
which three amino acids are considered aromatic ?
phenylalanine
tyrosine
trypyophan
which two amino acids are considered acidic?
aspartic acid
glutamic acid
which three amino acids are considered basic ?
histidine
lysine
arginine