Protein Kinases Flashcards
List the different Ser/Thr kinases.
- AGC
- CAMK
- CK1
- CMGC
- STE
- TKL
- RGC
- others
Kinases function as ____ based on conformational change.
switches
PKA
protein kinse A
With kinases, ATP is coordinated with ____ and ____.
Lys72; Asp184
Which amino acid binding allows for substrate hydroxyl group activation?
Asp166
When CDK activity is high, what does it do?
It allows for binding of cyclin which directs CDKs to substrates for phosphorylation.
Group of cytosolic tyrosine kinases that regulates cell adhesion and lymphocyte activation.
Src Family Kinases
For Src kinases, phosphorylation of ____ in the C-terminal is inhibitory.
Tyr527
Full activation of Src requires ____ of Tyr527 and ____ of Tyr416.
dephosphorylation; phosphorylation
Which kinase group contains PKA, PKG, PKC families.
AGC
Which kinase group contains calcium/calmodulin-dependent protein kinase?
CAMK
Which kinase group contains Casein kinase 1?
CK1
Which kinase group contains CDK, MAPK, GSK3, CLK families?
CMGC
Which kinase group contains homologs of yeast Sterile 7, Sterile 11, Sterile 20 kinases?
STE
Which kinase group conatins tyrosine kinase?
TK
Which kinase group contains tyrosine kinase-like?
TKL
Which kinase group contains receptor guanylate cyclases are a small ePK group similar in sequence to the Tyrosine Kinase group found in metazoa but absent from fungi, plants and protists?
RGC
The numbers following an amino acid are based on ____.
PKA (protein kinase A)
Proper positioning of residues (a.a.) in the binding pocket of protein kinases requires ____ interations with key elements.
tertiary
Cyclins directs ____ to proper substrates for phosphorylation.
CDKs
Cyclin-dependent kinase (CDK) activation is regulated by proper positioning of ____ and the ____ ____ (“regulatory lever”)
c-helix; activation loop
What is the most important regulatory element in phosphorylated CDK that is bound to cyclin?
the activation loop in position
What stabilized the active conformation of insulin receptor tyrosine kinase (IRK)?
activation-loop phosphorylation
What is the mechanism of substrate specificity in protein kinases?
a) the active site
b) “docking sites” that recogized motifs
c) accessory domains with protein-protein domain interaction
d) accessory subunit requirement as allosteric activator
Determine the form of substrate specificity.
active site
Determine the form of substrate specificity.
docking interactions
e.g. ser/thr kinases
Determine the form of substrate specificity.
modular domains
e.g. tyrosine kinases
Determine the form of substrate specificity.
scaffolds/adaptors
e.g. CDKs
