Protein Kinases

Question 1

List the different Ser/Thr kinases.

Answer 1

• AGC
• CAMK
• CK1
• CMGC
• STE
• TKL
• RGC
• others

Question 2

Kinases function as ____ based on conformational change.

Answer 2

switches

Question 3

PKA

Answer 3

protein kinse A

Question 4

With kinases, ATP is coordinated with ____ and ____.

Answer 4

Lys72; Asp184

Question 5

Which amino acid binding allows for substrate hydroxyl group activation?

Answer 5

Asp166

Question 6

When CDK activity is high, what does it do?

Answer 6

It allows for binding of cyclin which directs CDKs to substrates for phosphorylation.

Question 7

Group of cytosolic tyrosine kinases that regulates cell adhesion and lymphocyte activation.

Answer 7

Src Family Kinases

Question 8

For Src kinases, phosphorylation of ____ in the C-terminal is inhibitory.

Answer 8

Tyr527

Question 9

Full activation of Src requires ____ of Tyr527 and ____ of Tyr416.

Answer 9

dephosphorylation; phosphorylation

Question 10

Which kinase group contains PKA, PKG, PKC families.

Answer 10

AGC

Question 11

Which kinase group contains calcium/calmodulin-dependent protein kinase?

Answer 11

CAMK

Question 12

Which kinase group contains Casein kinase 1?

Answer 12

CK1

Question 13

Which kinase group contains CDK, MAPK, GSK3, CLK families?

Answer 13

CMGC

Question 14

Which kinase group contains homologs of yeast Sterile 7, Sterile 11, Sterile 20 kinases?

Answer 14

STE

Question 15

Which kinase group conatins tyrosine kinase?

Answer 15

TK

Question 16

Which kinase group contains tyrosine kinase-like?

Answer 16

TKL

Question 17

Which kinase group contains receptor guanylate cyclases are a small ePK group similar in sequence to the Tyrosine Kinase group found in metazoa but absent from fungi, plants and protists?

Answer 17

RGC

Question 18

The numbers following an amino acid are based on ____.

Answer 18

PKA (protein kinase A)

Question 19

Proper positioning of residues (a.a.) in the binding pocket of protein kinases requires ____ interations with key elements.

Answer 19

tertiary

Question 20

Cyclins directs ____ to proper substrates for phosphorylation.

Answer 20

CDKs

Question 21

Cyclin-dependent kinase (CDK) activation is regulated by proper positioning of ____ and the ____ ____ (“regulatory lever”)

Answer 21

c-helix; activation loop

Question 22

What is the most important regulatory element in phosphorylated CDK that is bound to cyclin?

Answer 22

the activation loop in position

Question 23

What stabilized the active conformation of insulin receptor tyrosine kinase (IRK)?

Answer 23

activation-loop phosphorylation

Question 24

What is the mechanism of substrate specificity in protein kinases?

Answer 24

a) the active site
b) “docking sites” that recogized motifs
c) accessory domains with protein-protein domain interaction
d) accessory subunit requirement as allosteric activator

Question 25

Determine the form of substrate specificity.

Answer 25

active site

Question 26

Determine the form of substrate specificity.

Answer 26

docking interactions

e.g. ser/thr kinases

Question 27

Determine the form of substrate specificity.

Answer 27

modular domains

e.g. tyrosine kinases

Question 28

Determine the form of substrate specificity.

Answer 28

scaffolds/adaptors

e.g. CDKs