Principles & Mechanisms of Protein Interactions Flashcards

1
Q

What is involved in protein-protein interaction?

A
  • 3-D conformation
  • Non-covalent physical interactions - ionic bonds, hydrogen bonds, Van der Waal’s interactions
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2
Q

What are the four levels of protein structure?

A

1) primary
2) secondary
3) tertiary
4) quaternary

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3
Q

They cytoplasm is densely packed with ____ which stabilize protein interactions.

A

macromolecules

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4
Q

What is the mechanism of protein-protein interactions influenced by?

A
  • concentrations of components (Km) values
  • dynamics of how rapidly the interactions form and break (dissociate and association rates) – Kd
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5
Q

List the signaling inputs that can alter the binding of proteins with one another.

A

1) protein concentration
2) distribution of the protein throughout the cell
3) changes in post-translational modification
4) Assembly of more complicated multifunctional structures

Changes in binding behaviors can transmit information

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6
Q

What is an example of a more built of structure which holds substrates in place?

A

scaffolds

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7
Q

What does this represent?

A

recruitment

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8
Q

What is the orange molecule in this picture and what does it do to the active protein?

A

The molecule is a ligand and it causes a conformational change

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9
Q

What are two type of protein-protein interaction surfaces?

A

a) broad surfaces
b) short, linear peptide sequence with groove

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10
Q

What kind of protein interaction surface is this?

A

broad surface

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11
Q

What kind of protein interaction surface is this?

A

short, linear peptide sequence in groove

SH2 domain-pY

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12
Q

Any segment of a polypeptide chain that can fold independently into a compact, stable structure.

A

protein domain

serves as modular unit

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