Principles & Mechanisms of Protein Interactions Flashcards
What is involved in protein-protein interaction?
- 3-D conformation
- Non-covalent physical interactions - ionic bonds, hydrogen bonds, Van der Waal’s interactions
What are the four levels of protein structure?
1) primary
2) secondary
3) tertiary
4) quaternary
They cytoplasm is densely packed with ____ which stabilize protein interactions.
macromolecules
What is the mechanism of protein-protein interactions influenced by?
- concentrations of components (Km) values
- dynamics of how rapidly the interactions form and break (dissociate and association rates) – Kd
List the signaling inputs that can alter the binding of proteins with one another.
1) protein concentration
2) distribution of the protein throughout the cell
3) changes in post-translational modification
4) Assembly of more complicated multifunctional structures
Changes in binding behaviors can transmit information
What is an example of a more built of structure which holds substrates in place?
scaffolds
What does this represent?
recruitment
What is the orange molecule in this picture and what does it do to the active protein?
The molecule is a ligand and it causes a conformational change
What are two type of protein-protein interaction surfaces?
a) broad surfaces
b) short, linear peptide sequence with groove
What kind of protein interaction surface is this?
broad surface
What kind of protein interaction surface is this?
short, linear peptide sequence in groove
SH2 domain-pY
Any segment of a polypeptide chain that can fold independently into a compact, stable structure.
protein domain
serves as modular unit