Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

QUIZ 2 > Protein IV > Flashcards

Protein IV Flashcards

1
Q

dialysis

A

separates based on size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

dialysis elution

A

large molecules retained, small molecules diffuse out

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

centrifugation

A

separates based on size and density

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

centrifugation elution

A

larger, more dense proteins sediment faster

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

size exclusion (gel filtration) chromatography

A

separates based on size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

size exclusion chromatography elution

A

large proteins elute first, small proteins elute last; small molecules get stuck in porous beads

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

anion exchange chromatography

A

separates based on negative charge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

anion exchange elution

A

positively charged proteins will not bind and elute first; most negatively charged elute last

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

pH < pKa

A

positive charge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

cation exchange chromatography

A

separates based on positive charge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

cation exchange elution

A

negatively charged proteins do not bind and elute first; the most positive will elute last

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

affinity chromatography

A

separates based on specific interaction with protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

affinity elution

A

only specific protein will bind to column

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

HPLC

A

higher resolution, better separation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

gel electrophoresis

A

separates based on size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

gel electrophoresis elution

A

small DNA molecules run faster than large molecules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

SDS-PAGE

A

denatures protein for uniform charge; separates based on size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

SDS-PAGE elution

A

small proteins faster than large

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

isoelectric focusing

A

separates based on pKa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

isoelectric focusing elution

A

proteins with highest pKa run closest to negative electrode; proteins with lowest pKa run closest to positive electrode

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

western blot

A

detects specific proteins

22
Q

western blot info

A

proteins are separated by SDS-PAGE prior to blot

23
Q

ELISA

A

detects specific proteins

24
Q

ELISA info

A

proteins are not separated

25
Q

peptide fingerprinting

A

detects peptides from proteolytic digestion of protein

26
Q

peptide fingerprinting info

A

separates based on size in one direction and charge in the other

27
Q

edman degradation

A

detects amino acid sequence of protein

28
Q

mass spectrometry

A

detects molecular mass of protein; can compare to known proteins in database to find what the protein is

29
Q

NMR spectrometry

A

detects protein structure and dynamics

30
Q

x-ray crystallography

A

detects protein structure

31
Q

cryo-electron microscopy

A

detects structure of protein complexes

32
Q

dialysis in medicine

A

in renal failure, urea and other small solutes accumulate in the blood –> dialysis is used to remove the small solutes (they can cross dialysis membrane, but blood cells will stay put)

33
Q

the greater the sedimentation coefficient _

A

the faster particles will sediment

34
Q

centrifugation in blood

A

RBCs will sediment faster, resulting in red blood cells at bottom and plasma at top

35
Q

predicting elution in size exclusion

A

weight is determined by total MW (not just subunit) –> a 56.6 tetramer is actually 226.4

36
Q

anion exchange setup

A

column is loaded with positively charged anions to trap negative proteins; to displace these bound negative proteins and allow them to elute, Cl- is added

37
Q

cation exchange setup

A

column beads are negatively charged to trap positive proteins; Na+ is added to displace the bound positive proteins and allow them to elute

38
Q

pH < pI

A

net positive charge

39
Q

pH > pI

A

net negative charge

40
Q

isoelectric focusing in medicine

A

sickle cell hemoglobin has higher isoelectric point than regular

41
Q

western blotting setup

A

SDS-PAGE separates proteins –> transfer to nitrocellulose –> sheet incubated with an antibody that recognizes proteins of interest

42
Q

ELISA

A

similar to Western blotting but proteins are bound to a solid support

43
Q

direct ELISA

A

reporter enzyme is coupled directly to the primary antibody

44
Q

indirect ELISA

A

reporter enzyme is coupled to secondary antibody

45
Q

capture assay ELISA

A

an antibody is bound to the solid surface then the antigen is added –> to detect antigen a primary antibody is added –> excess antibody is washed off –> secondary antibody is added –> washed off –> color reaction

46
Q

polyclonal antibodies

A

mixture of several antibodies that are usually produced by different B cell clones of an animal

47
Q

monoclonal antibodies

A

antibodies generated from identical B cells that recognize a single epitope

48
Q

polyclonal antibody formation

A

inject antigen into an animal –> after immunization, polyclonal antibodies can be obtained directly from serum

49
Q

monoclonal antibody formation

A

B-lymphocytes are isolated from the animal’s spleen and fused with a myeloma cell line –> immortal B-cell-myeloma-hybridomas –> grow continuously in culture producing antibodies

50
Q

in isoelectric focusing, as pH increases _

A

proteins with low pIs will stop moving; those with highest pI’s will stop moving at highest pH

QUIZ 2 (10 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions