PROKARYOTIC REPLICATION to ANTIMETABOLITES Flashcards
verifies if base pairing is correct and catalyzes formation of new phosphodiester linkage between nucleotide and growing strand
DNA POLYMERASE III
influence the unwinding of DNA double helix and hydrogen bond between complementary bases are broke
DNA HELICASE
biochemical process which DNA molecules produce exact duplicates of themselves
DNA REPLICATION
Steps of prokaryotic replication of DNA molecules
1) Helicases unwind
2) Single-strand binding proteins
3) leading strand
4) lagging strand
5) After the RNA primer
point which DNA double helix unwinds and is constantly changing/moving
REPLICATION FORK
strand grows continuously
LEADING STRAND
strand is synthesized small segments
LAGGING STRAND
segments, the DNA unwinds
OKAZAKI FRAGMENTS
Okazaki fragments is named after
REIJI OKAZAKI
breaks or gaps of the daughter strand
NICKS
influence the unwinding of DNA double helix and hydrogen bond between complementary bases are broke
DNA HELICASE
recognizes primer and begins to extend it with DNA
DNA POLYMERASE III
synthesizes short stretches of RNA (10 nucleotides long) that are completely complementary and antiparallel to DNA template
PRIMASE
RNA prime an initiates DNA synthesis
RNA PRIMER
main ingredient of replication
RNA PRIMER
used for PCR amplification
DNA PRIMER
PCR meaning
polymerase chain reaction
removes RNA primers from fragments and replace the required nucleotides
DNA POLYMERASE I
connects two strands of DNA together by forming a bond between phosphate group of one strand (DNA pol. III) and deoxyribose grp on another (DNA pol. I)
DNA LIGASE
used in cells to join together Okazaki fragments which are formed in lagging strand
DNA LIGASE
zips old and new strands together
DNA LIGASE
it removes primer
EXONUCLEASE
this material unwinds the DNA
DNA helicase
these materials manufacture new DNA strands
POLYMERASE
