Profame - Chloride

Question 1

METHOD:

Answer 1

Schoenfeld & Lewellen

Question 2

Chloride ions in serum displaces ____ from the ______forming _______ which is only very slightly ionized.

Answer 2

thiocyanate ions from the mercuric thiocyanate forming mercuric chloride

Question 3

Color of the thiocyanate complex

Answer 3

orange-yellow

Question 4

Wavelength

Answer 4

480 - 492 nm

Question 5

Sub wavelength

Answer 5

550 nm

Question 6

Conversion:

Answer 6

1 = mEq/1

Question 7

NORMAL RANGE:

Answer 7

SERUM: 96 - 110 mmol/l
CSF : 125 - 135 mmol/l
URINE: 170 - 255 mmol/l (24Hrs urine sample)

Question 8

NR:

Answer 8

SERUM: 96 - 110 mmol/l
CSF :125 - 135 mmol/l
URINE: 170 - 255 mmol/l (24Hrs urine sample)

Question 9

Principle

Answer 9

Chloride ions in serum displaces thiocyanate ions from the mercuric thiocyanate forming mercuric chloride which is only very slightly ionized. The liberated thiocyanate ions reacts with ferriions to form the orange-yellow thiocyanate complex which is measured at 480-492 nm.