Lab: Icterus Index

Question 1

Who is the author of icterus index

Answer 1

Meulengracht (Hilado, 1996)

Question 2

Objective of the icterus index

Answer 2

To learn the method and technical of determining serum icterus index.

Question 3

Dilution factor

Answer 3

1:10

Question 4

Principle

Answer 4

The intensity of the golden yellow color imparted to the serum by bilirubin is measured by comparing the serum color with the color of the standard solution.

Question 5

Standard solution

Answer 5

Potassium dichromate

Question 6

Blank

Answer 6

Distilled water

Question 7

One unit of II has been defined as the color of a ________ solution to potassium dichromate

Answer 7

1:10,000

Question 8

Buffer solution

Answer 8

Phosphate buffer solution

Question 9

Ph of phosphate buffer solution

Answer 9

7.4

Question 10

Wavelength

Answer 10

415 nm

Question 11

If the reading is high, transfer to ___ of the mixture to another test tube and dilute to ___ with ____.

Answer 11

1 ml
10 with distilled water

Question 12

It is essential that the serum for the II determination is absolutely free of visible

Answer 12

Hemolysis and chyle

Question 13

The patient should not eat food items which contain yellow pigment for _____ hours prior to the drawing of blood for the test.

Answer 13

14-48 hrs

Question 14

Normal values

Answer 14

4-6 units

Question 15

Preparation of reagents:

Standard stock solution

Answer 15

1. Dissolve 1 gm of potassium dichromate in 90 ml of distilled water.
2. Add 0.1 ml of concentrated sulfuric acid and dilute to 100 ml.
3. Keep in brown bottle.

Question 16

Preparation of reagents:

Standard working solution

Answer 16

Dilute 10 ml of stock standard solution to 1L with distilled water.

Question 17

Preparation of reagents:

Phosphate buffer solution, pH 7.4

Answer 17

1. Into a flask, place 23.16 gm monopotassium dihydrogen phosphate.
2. Add 400 ml distilled water and dissolve
3. Add 4.8 gm of sodium hydroxide reagent grade.
4. Dissolve
5. Check pH
6. Transfer with rinsings to a 500 ml volumetric flask
7. Dilute to the graduation mark
8. Store in refrigerator