Direct Bilirubin Flashcards
Intended use
Diagnostic reagent for quantitative in vitro determination of direct bilirubin in human serum or heparin plasma on automated photometric systems
Method
Photometric test using 2,4-dichloroaniline (DCA)
Direct bilirubin in presence of diazotized 2,4-dichloroaniline forms a red colored azocompound in acidic solution.
Components and Concentrations of Reagent 1
EDTA-Na2 - 0.1 mmol/L
NaCl - 150 mmol/L
Sulfamic acid - 100 mmol/L
Components and Concentrations of Reagent 2
2,4-Dichloroaniline - 0.5 mmol/L
HCl - 900 mmol/L
EDTA-Na2 - 0.13 mmol/L
Warnings and Precautions of Reagent 1 & 2:
H290 - may be corrosive to metals
P234 - keep only in original packaging
P390 - absorb spillage to prevent material damage
Other warnings and precautions:
- In very rare cases, samples of patients with gammopathy might give falsified results
- Eltrombopag medication leads to falsely low or high results in patient samples.
- Please refer to the safety data sheets and take the necessary precautions for the use of laboratory reagents. For diagnostic purposes, the results should always be assessed with the patient’s medical history, clinical examinations and other findings
Specimen
Human serum or heparin plasma
Stability of specimen:
2 days at 20 – 25°C
7 days at 4 – 8°C
6 months at –20°C
in case of immediate freezing.
Assay Procedure:
Wavelength
545/658 nm
Assay Procedure:
Temperature
37°C
Assay Procedure:
Measurement
Endpoint
Assay Procedure:
Sample/Calibrator
3.5 µL
Assay Procedure:
Reagent 1
80 µL
Assay Procedure:
Reagent 2
20 µL
Assay Procedure:
Addition reagent 2
Cycle 19 (286 s)
