AST

Question 1

Components and Concentrations:

R1

Answer 1

TRIS (pH 7.65) - 110 mmol/L
L-Aspartate - 320 mmol/L
MDH (malate dehydrogenase) - >_ 800 U/L
LDH (lactate dehydrogenase) - >_ 1200 U/L

Question 2

Components and concentrations

R2

Answer 2

2-Oxoglutarate - 85 mmol/L
NADH - 1 mmol/L

Question 3

Components and Concentrations:

Pyridoxal-5-phosphate

Answer 3

Good’s buffer (pH 9.6) - 100 mmol/L
Pyridoxal-5-phosphate - 13 mmol/L

Question 4

Warnings and precautions:

Reagent 1

Answer 4

Contains animal and biological material

Question 5

Warnings and precautions:

Reagent 2

Answer 5

Contains biological material

Question 6

Warnings and precautions:

Preservative of the reagents

Answer 6

Sodium azide (0.95 g/L)

Question 7

Wavelength

Answer 7

340/410 nm

Question 8

Measurement:

Answer 8

Kinetic

Question 9

Sample/Calibrator

Answer 9

6.0 uL

Question 10

Reagent 1

Answer 10

80 uL

Question 11

Reagent 2

Answer 11

20 uL

Question 12

Conversion factor

Answer 12

0.0167 ukat/L

Question 13

Measuring range up to

Answer 13

600 U/L

Question 14

Limit of detection

Answer 14

1.2 U/L

Question 15

Interfering substances (with and without P-5-P)

Answer 15

Ascorbic acid - 30 mg/dL
Bilirubin - 60 mg/dL
Hemoglobin - 100 mg/dL
Lipemia - 200 mg/dL

Question 16

Reference range:

Women

Answer 16

<31 U/L
< 0.52 ukat/L

Question 17

Reference range:

Men

Answer 17

<35 U/L
<0.58 ukat/L