ALP

Question 1

Alkaline phosphatase originate mainly from

Answer 1

Bone and liver

Question 2

ALP is also from other tissues such as

Answer 2

Kidney, placenta, testes, thymus, lung and tumors

Question 3

Physiological increases are found during

Answer 3

Bone growth in childhood
Pregnancy

Question 4

Pathological increases are largely associated with

Answer 4

Hepatobiliary and bone diseases

Question 5

In hepatobiliary disease they indicate obstruction of the bile ducts as in ______ caused by

Answer 5

Cholestasis

Caused by: gall stones, tumors or inflammation

Question 6

Elevated activities are also observed in

Answer 6

Infectious hepatitis

Question 7

In bone diseases elevated AP activities originate from increased osteoblastic activity as in

Answer 7

Paget’s disease, osteomalacia (rickets), bone metastases and
hyperparathyroidism.

Question 8

Method

Answer 8

Kinetic photometric test, according to the International Federation of
Clinical Chemistry and Laboratory Medicine (IFCC)

Question 9

Components and concentrations of Reagent 1

Answer 9

2-Amino-2-methyl-1-propanol
Magnesium acetate
Zinc sulphate
HEDTA

Question 10

Components and concentrations of Reagent 2

Answer 10

p-Nitrophenylphosphate

Question 11

Sample Start

Answer 11

Mix 4 parts of R1 + 1 part of R2

Question 12

Stability of the reagent

Answer 12

4 weeks at 2 – 8°C
5 days at 15 – 25°C

Question 13

Specimen

Answer 13

Serum or heparin plasma

Question 14

Wavelength

Answer 14

Hg 405 nm, 400 – 420 nm

Question 15

Optical path

Answer 15

1 cm

Question 16

Temperature

Answer 16

37°C

Question 17

Measurement

Answer 17

Against reagent blank

Question 18

Conversion Factor

Answer 18

0.0167 = AP [µkat/L]

Question 19

recommended for calibration.

Answer 19

DiaSys TruCal U

Question 20

Measuring range up to

Answer 20

1400 U/L.

Question 21

Limit of detection

Answer 21

0.6 U/L

Question 22

Onboard stability

Answer 22

6 days

Question 23

Calibration stability

Answer 23

6 days

Question 24

Interfering substance

Answer 24

Ascorbic acid
Bilirubin (conjugated)
Bilirubin (unconjugated)
Hemoglobin
Lipemia (triglycerides)

Question 25

Reference Range

Answer 25

Women 35 – 104 [U/L] 0.58 – 1.74 µkat/L
Men 40 – 129 [U/L] 0.67 – 2.15 µkat/L