Process of getting insulin Flashcards
1
Q
What is the first step?
A
Insulin gene is cut out of human DNA
2
Q
What is the second step?
A
Plasmid is isolated from the bacteria and cut open
3
Q
What is the third step?
A
2 pieces of DNA are glued together making recombinant plasmid
4
Q
What is the fourth step?
A
New recombinant plasmid is put back into a bacterial cell
5
Q
What is the fifth step?
A
Bacteria reproduces in culture and starts making new protein (insulin)
6
Q
What is the sixth step?
A
Extract and purify insulin
