Pretransfusion Testing/Donor Selection and Phlebotomy

Question 1

procedure to provide the safest blood possible for transfusion

Answer 1

compatibility testing

Question 2

most fatal transfusion reactions

Answer 2

clerical errors

Question 3

required tests on DONOR units

Answer 3

ABO/D
antibody screen
infectious diseases

Question 4

required tests on RECIPIENT

Answer 4

ABO/D

antibody screen

Question 5

unit of blood to transfuse in case of EMERGENCIES

Answer 5

“O” Rh negative blood units (esp to women within child bearing age)
“O” Rh positive for males and women BEYOND child bearing age

Question 6

one unit of RBC should INCREASE

Answer 6

hemoglobin by 1 g/dl

hematocrit by 3%

Question 7

units that don’t need crossmatching

Answer 7

plasma, platelet concentrates, cryoprecipitate

Question 8

units that might need crossmatching

Answer 8

platelet apheresis and granulocyte concentrates (contain more than 2mL of red blood cells)

Question 9

3 phases of donor screening

Answer 9

registration
health history review
physical examination

Question 10

number of weeks between whole blood donation

Answer 10

8 weeks or more

Question 11

number of weeks between 2-unit red cell collection

Answer 11

16 weeks or more

Question 12

number of weeks for infrequent plasmapheresis donors

Answer 12

4 weeks or more

Question 13

number of days for frequent plasmapheresis, plateletpheresis and leukapheresis donors

Answer 13

2 days or more

Question 14

length of capillary tube

Answer 14

70mm

Question 15

bore of capillary tube

Answer 15

1mm

Question 16

acceptable hemoglobin

Answer 16

> 12.5g/dl

Question 17

acceptable hematocrit

Answer 17

> 38%

Question 18

acceptable blood pressure

Answer 18

<180/100 mmHg (S/D)

Question 19

acceptable temperature

Answer 19

<37.5C (99.5F)

Question 20

acceptable pulse

Answer 20

50-100 beats per minute

but if athlete: <50 is OK

Question 21

acceptable age

Answer 21

16-65 y/o

Question 22

storage conditions of normovolemic hemodilution

Answer 22

room temperature for 8 hours

1-6C for 24 hours

Question 23

cleaning of venipuncture site

Answer 23

0.7% aqueous iodophore then 10% povodine iodine
4cm in all directions with a minimum 30 seconds contact time
alternative: chlorhexidine and 70% isopropyl alcohol

Question 24

gauge of needle

Answer 24

16g

Question 25

acceptable donation time

Answer 25

8-12 minutes

>15 minutes would disqualify unit for platelet and cryoprecipitate preparation

Question 26

weight of a 405-550 ml unit

Answer 26

429-583 g

Question 27

most common adverse donor reaction

Answer 27

hematoma

Question 28

if the donor is experiencing spasm and twitching, you should:

Answer 28

have the donor cough

Question 29

acceptable hemoglobin for autologous blood donation

Answer 29

11 g/dl

Question 30

acceptable hematocrit for autologous blood donation

Answer 30

33%

Question 31

autologous blood can be collected

Answer 31

72 hours or more before the surgery

Question 32

tests done on autologous blood units

Answer 32

ABO/D

unexpected antibodies

Question 33

duration time of apheresis

Answer 33

30 minutes to 2 hours

Question 34

requirements for red cell apheresis (2-unit red cell units)

Answer 34

male donors: at least 130 lbs, min. 5’1”

female donors: at least 150 lbs, min. 5’5”