PRESUMPTIVE IDENTIFICATION OF GRAM-POSITIVE ANAEROBES

Question 1

Large, flat colonies; barnyard odor,
chartreuse fluorescence

Answer 1

Clostridium difficile

Question 2

Thin rods, rare spores

Answer 2

CELLULAR MORPHOLOGY OF Clostridium difficile

Question 3

Boxcar, large, square rods

Answer 3

CELLULAR MORPHOLOGY OF C. perfringens

Question 4

Thin rods, subterminal spores

Answer 4

CELLULAR MORPHOLOGY OF C. septicum

Question 5

Thin rods, subterminal spores

Answer 5

CELLULAR MORPHOLOGY OF C. sordellii

Question 6

Smoothly swarming

Answer 6

C. septicum

Question 7

Very large, lobate, irregular, flat

Answer 7

C. sordellii

Question 8

Smoothly swarming but slow growing

Answer 8

C. tetani

Question 9

Small, peaked, circular

Answer 9

“Peptostreptococci”

Question 10

Small, opaque, enamel white, circular
(catalase-positive)

Answer 10

Cutibacterium acnes

Question 12

Cocci, pairs and chains

Answer 12

CELLULAR MORPHOLOGY OF “Peptostreptococci”

Question 13

Coryneform rods

Answer 13

CELLULAR MORPHOLOGY OF Cutibacterium acnes

Question 14

Swollen terminal spores

Answer 14

CELLULAR MORPHOLOGY OF C. tetani

Question 15

C. sordellii
C. tetani
Cutibacterium acnes

Answer 15

Positive for SPOT
INDOLE

Question 16

Clostridium difficile
C. septicum

Answer 16

Negative for SPOT
INDOLE

Question 17

C. perfringens
“Peptostreptococci”

Answer 17

Not done for SPOT
INDOLE