Prepping stained temporary mount - Cheek cells

Question 1

What is the method?

Answer 1

1. Put dot in middle of a slide with marker + turn so mark is on lower side - Helps locate cheek cells
2. Use cotton bud to remove cheek cells
3. Rub cotton bud on slide to make smear + dispose of it
4. Add 2 drops of methylene blue to smear
5. Use mounted needle to lower cover slip. Blot any excess with paper towel
6. Observe smear under microscope

Question 2

What are the advantages of using temporary mounts?

Answer 2

Easy to add stain for contrast, quick to prepare

Question 3

What is the disadvantage of using temporary mounts?

Answer 3

Dries up quickly so only temporary