Effect of substrate conc on enzyme activity

Question 1

What is the method?

Answer 1

1. Grind potato piece with distilled water to make paste including enzyme
2. Put hydrogen peroxide (H2O2) in test tube
3. Using forceps, dip filter paper in enzyme suspension (potato paste), tap off excess
4. Put paper in H2O2 solution
5. Measure time taken for paper to sink + float again

Question 2

What are the hazards?

Answer 2

Hydrogen peroxide, blender + knife, beaker

Question 3

Whats the risk of hydrogen peroxide + how to avoid?

Answer 3

Oxidising agent - damage to tissue

Wear goggles + gloves, report spillages

Question 4

Whats the risk of blender + knife, and how to avoid?

Answer 4

Sharp edges can create puncture wound

Follow safe procedures, cut away from body

Question 5

Whats the risk of beakers + how to avoid?

Answer 5

Glass can break

Dont put on edge of table

Question 6

Whats the IV?

Answer 6

Conc of H2O2

Question 7

Whats the DV?

Answer 7

Time taken for disc to sink + float

Question 8

Whats the CV?

Answer 8

Same amount of potato, temp, overall volume of H2O2