PRELIM LABORATORY L1: RBC SUSPENSION

Question 1

reagents used in RCS preparation

Answer 1

EDTA blood sample
0.85-0.90% NSS

Question 2

percent required of RCS

Answer 2

3-5%

Question 3

concentration of heavy suspension

Answer 3

5%

Question 4

in heavy suspension, is antigen suspension high or low?

Answer 4

high

Question 5

heavy suspension may result into what phenomenon

Answer 5

post-zone

Question 6

what is post-zone phenomenon?

Answer 6

excess of antigen leading to a false-negative result

Question 7

concentration of light suspension

Answer 7

<2 or 3%

Question 8

in light suspension, is antigen suspension high or low?

Answer 8

low

Question 9

light suspension may result into what phenomenon

Answer 9

prozone

Question 10

what is prozone phenomenon?

Answer 10

excess antibody and insufficient antigen concentration leading to a false-negative result

Question 11

how is prozone remedied?

Answer 11

dilution

Question 12

RCS is used for what procedures (4)

Answer 12

ABO and Rh typing
Direct Antiglobulin Test and Auto control
Donor unit compatibility
Red Cell Phenotyping

Question 13

Why can’t water be used as instead of NSS?

Answer 13

water can hemolyze rbcs

Question 14

how many times is washing step repeated?

Answer 14

3 times

Question 15

formula for RCS

Answer 15

%RCS = (PCV/TV)x 100

Question 16

how long is prepared RCS viable?

Answer 16

1 week

Question 17

temperature for storage of RCS

Answer 17

1-6 C

Question 18

expected color of RCS

Answer 18

tomato red