LAB: ANTIBODY TITRATION Flashcards
what is antibody titration
semi-quantitative method to measure the concentration of a circulating antibody present in px serum/plasma
purpose of antibody titration
measure the concentration of antibodies
determine the reactivity of antibodies
antibody titration utilizes what principle
IAT
clinical application of antibody titration studies
1) estimating antibody activity among pregnant women
2) screening of blood products (platelets/plasma) to determine risk of HTR
3) elucidating autoantibody specificity
4) characterizing high titer low avidity (HTLA) antibodies
5) studying the effect of sulfhydryl reagents on antibody behavior to determine immunoglobulin type
6) testing for antibodies for preparation and manufacturing of reagents
procedure involved in preliminary phase of antibody titration
1) perform antibody screening and identification
2) prepare 10 test tubes
3) label the tubes as tt1 to tt10
4) perform two-fold serial dilution
5) proceed to antibody titration
procedure under antibody titration
1) prepare another set of 10 tubes
2) label as A1 to A10
3) transfer 2 drops of each diluted serum to respective tubes
3) add 1 drop of reagent screening cells
4) IS phase
5) incubation phase
6) AHG phase
7) check cells
which reagent screening red cells should be used
the most reactive
how to obtain the antibody titer
getting the reciprocal of the highest serum dilution showing positive reaction
identify the score of Ab strength:
4+
12
identify the score of Ab strength:
3+
10
identify the score of Ab strength:
2+
8
identify the score of Ab strength:
1+
5
identify the score of Ab strength:
+/- or w+
2
identify the score of Ab strength:
0
0
in serum dilution, what may be used as a diluent
NSS or albumin
if albumin is used as diluent, what should be done to reagent screening red cells
must also be suspended in albumin
how much increase in antibody titer is considered clinically significat
four-fold (two tube dilution)
T or F:
hemolysis show a positive reaction and can be quantified
F
cannot be quantified
alternative to 5% rcs to achieve optimal ratio
mix 2 drops if only 2% rcs is used
how many drops of serum are added to antibody titration
2 drops
