LAB: BROAD SPECTRUM COMPATIBILITY TEST Flashcards
series of procedures designed to ensure the safety of blood for transfusion
compatibility testing
performed prior to the transfusion of blood components containing red blood cells
crossmatching
2 parts of crossmatching
major crossmatch
minor crossmatch
what does major crossmatch consists
mixing the px serum with donor red cells (PS-DR)
what does minor crossmatch consists
mixing the donor’s serum with the px red cells (PR-DS)
autocontrol consists of
px red cells and px serum
M label stands for
major crossmatch
MA label stands for
major crossmatch w/ albumin
N label stands for
minor crossmatch
NA label stands for
minor crossmatch w/ albumin
immediate saline spin is aka
protein/albumin/room temperature phase
how many seconds are the tubes centrifugated in immediate spin
15 s
if no agglutination or hemolysis is observed, what tubes will proceed to the thermo phase
MA and NA
what reagent can be used instead of 22% bovine serum albumin if the tubes proceeding to the next step will be incubated for 10 mins
LISS
incubation phase is aka
thermo phase
MA and NA tubes are incubated for how many minutes
30 minutes
how many minutes are the tubes incubated if LISS was used
10 minutes
proceed to AHG phase if
no agglutination or hemolysis is observed in MA and NA tubes
at what phase is check cells reagent added
AHG phase
how many times are the cells washed in AHG phase
3 times
how many drops of AHG sera are added
2 drops
in AHG phase, tubes are centrifuged for how many seconds
15 seconds
how many drop of check cells are added
1 drop
interpret:
no agglutination or hemolysis in all tubes
compatible
interpret:
agglutination or hemolysis in any tubes
incompatible
Blood is compatible in major crossmatch but incompatible in minor crossmatch. What should be done?
Blood can be transfused but with caution
contents of M tube
2 drops px serum, 1 drop 2-5% suspension of donor’s cells
contents of MA tube
2 drops px serum, 1 drop 2-5% suspension of donor’s cells
2 drops of 22% BSA
contents of N tube
2 drops of donor’s serum
1 drop of 2-5% suspension of px cells
contents of NA tube
2 drops of donor’s serum
1 drop of 2-5% suspension of px cells
2 drops of BSA
