PRE LAB ACT 3 AND 4 Flashcards
WHAT IS THE SIZE OF A GOOD SMEAR
2/3 or 3/4 of the slide
WHAT IS THE SIZE OF A GOOD SMEAR
2/3 or 3/4 of the slide
shape of a good smear
finger shaped with a slightly rounded at the feather edge
is a smooth witout irregulatities, holes and streak a good smear
yes
what is the appearance of the feather edge of the film
rainbow
fixations in order
methanol-eosin-methylene-water
what is the fizative in fixation
methanol
what is the negatively charge chemical in fixation
eosin
positively charged stain
Methylene
other name for spreader slide method
2 glass method
size of a slide
75 mm by 25 mm
angle of the spreader to the slide
30-45*
what is the diameter of the drop of blood
2-3mm
what does PASS stands for
Pressure, Angle, Speed, Size of blood
high hematocrit is indicative of
polycythemia
high hct increase or decrease
increase
what is seen on LPO
fibrin strand
what is fibrin strand
presence of coagulation
magnifiction of lpo,hpo,oio
10x,40x,100x
anticoagulant in lpo
EDTA
what is being viewed on hpo
wbc estimate
what magnification is roleaux formation observed
HPO
WHERE will you count for the platelet
in an area where RBCs has few overlapping
how many immersion fields are counted
10
what is the standard factor in plt estimation
20,000
result if there is 8-20 OIF
adequate plt ct.
what is the volume correction factor
XI.I
0<5/oif
expect lower plt count
result if >20/oif
expect higher plt ct.
this is the clumping of plt around wbc
plt satellitism
needle length in bleeding time
2mm
standard pressure in bleeding time
40 mmHg
bleeding time is the screening test for
- vascular disorder
- plt fxn defect
- detects problem in primary hemostasis
Plt adhesion is affected by
Calcium, ADP , fibrinogen
What activates plt
Thromboxane A2
What does cyclooxygenase activate
Thromboxane A2
Aspirin activates
Cyclooxygenase
Puncture is done on the earlobe
Duke method
Reference value for duke method
1-3 mins
Duke method is ideally for
Infants
What method is done on the forearm
Ivy method
Reference value for ivy mtd
1-7 mins
Uses template containing standardized slit
Template BT
Size of template BT
11 mm long, 1 mm deep
What are the blood thinners that affects IVy
Heparin, caumadin
Average of platelet
8-20 platelets