PRE LAB ACT 3 AND 4 Flashcards

1
Q

WHAT IS THE SIZE OF A GOOD SMEAR

A

2/3 or 3/4 of the slide

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2
Q

WHAT IS THE SIZE OF A GOOD SMEAR

A

2/3 or 3/4 of the slide

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3
Q

shape of a good smear

A

finger shaped with a slightly rounded at the feather edge

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4
Q

is a smooth witout irregulatities, holes and streak a good smear

A

yes

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5
Q

what is the appearance of the feather edge of the film

A

rainbow

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6
Q

fixations in order

A

methanol-eosin-methylene-water

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7
Q

what is the fizative in fixation

A

methanol

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8
Q

what is the negatively charge chemical in fixation

A

eosin

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9
Q

positively charged stain

A

Methylene

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10
Q

other name for spreader slide method

A

2 glass method

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11
Q

size of a slide

A

75 mm by 25 mm

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12
Q

angle of the spreader to the slide

A

30-45*

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13
Q

what is the diameter of the drop of blood

A

2-3mm

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14
Q

what does PASS stands for

A

Pressure, Angle, Speed, Size of blood

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15
Q

high hematocrit is indicative of

A

polycythemia

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16
Q

high hct increase or decrease

A

increase

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17
Q

what is seen on LPO

A

fibrin strand

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18
Q

what is fibrin strand

A

presence of coagulation

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19
Q

magnifiction of lpo,hpo,oio

A

10x,40x,100x

20
Q

anticoagulant in lpo

A

EDTA

21
Q

what is being viewed on hpo

A

wbc estimate

22
Q

what magnification is roleaux formation observed

A

HPO

23
Q

WHERE will you count for the platelet

A

in an area where RBCs has few overlapping

24
Q

how many immersion fields are counted

A

10

25
Q

what is the standard factor in plt estimation

A

20,000

26
Q

result if there is 8-20 OIF

A

adequate plt ct.

27
Q

what is the volume correction factor

A

XI.I

28
Q

0<5/oif

A

expect lower plt count

29
Q

result if >20/oif

A

expect higher plt ct.

30
Q

this is the clumping of plt around wbc

A

plt satellitism

31
Q

needle length in bleeding time

A

2mm

32
Q

standard pressure in bleeding time

A

40 mmHg

33
Q

bleeding time is the screening test for

A
  1. vascular disorder
  2. plt fxn defect
  3. detects problem in primary hemostasis
34
Q

Plt adhesion is affected by

A

Calcium, ADP , fibrinogen

35
Q

What activates plt

A

Thromboxane A2

36
Q

What does cyclooxygenase activate

A

Thromboxane A2

37
Q

Aspirin activates

A

Cyclooxygenase

38
Q

Puncture is done on the earlobe

A

Duke method

39
Q

Reference value for duke method

A

1-3 mins

40
Q

Duke method is ideally for

A

Infants

41
Q

What method is done on the forearm

A

Ivy method

42
Q

Reference value for ivy mtd

A

1-7 mins

43
Q

Uses template containing standardized slit

A

Template BT

44
Q

Size of template BT

A

11 mm long, 1 mm deep

45
Q

What are the blood thinners that affects IVy

A

Heparin, caumadin

46
Q

Average of platelet

A

8-20 platelets